166,671 results

1. pCas9

   Plasmid

   #42876

   Purpose

   Bacterial expression of Cas9 nuclease, tracrRNA and crRNA guide

   Depositor

   Luciano Marraffini

   Article

   Jiang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   tracr/Cas9
   

   Use

   CRISPR; E.coli

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. Toronto KnockOut version 3 library (TKOv3)

   Pooled Library

   #90294

   Purpose

   Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

   Depositor

   Jason Moffat

   Article

   Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available Since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcs2-GFP-IRES-mCherry

   Plasmid

   #226098

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. BPK1520

   Plasmid

   #65777

   Purpose

   Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SpCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.2-Nanoluc-ccdB

   Plasmid

   #87078

   Purpose

   Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

   Depositor

   Mikko Taipale

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Nanoluc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19

   Plasmid

   #50005

   Purpose

   pUC cloning vector

   Depositor

   Joachim Messing

   Article

   Norrander et al Gene. 1983 Dec;26(1):101-6.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-No-HaloTag7-mGFP-LC3

   Plasmid

   #184901

   Purpose

   Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   LC3B
   

   Use

   Tags

   HaloTag7 and monomeric EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP12251-pAAV-AiE0453m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2251)

    Plasmid

    #164453

    Purpose

    AiE0453m is an enhancer sequence designed to drive AAV-mediated transgene expression in L5_ET cortical neurons

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available Since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Wnt promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE80L-IL2 C125S

    Plasmid

    #104348

    Purpose

    Expresses human IL-2 (C125S)

    Depositor

    Alexander McLellan

    Insert

    Human IL-2 (C125S) (IL2 Human)
    

    Use

    Tags

    MRGSHHHHHGS-tag

    Expression

    Bacterial

    Mutation

    C125S mutation (TGT -> TCC)

    Promoter

    T5

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-mTagBFP2

    Plasmid

    #216126

    Purpose

    Lentiviral vector for expressing mTagBFP2.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-mTagBFP2-WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. prDNA spacer-LacOx120-20xMS2-Hyg

    Plasmid

    #210220

    Purpose

    Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Inserts

    120xLacO repeats derived from: PMID: 12864855.
    24xMS2 repeats derived from Addgene plasmid # 31865
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP1011-pAAV-mscRE4-minBGpromoter-tTA2-WPRE-hGHpA

    Plasmid

    #163480

    Purpose

    Direct-expressing tTA2 AAV Virus. Alias: AiP1011 - pAAV-AiE2004m-minBG-tTA2-WPRE-HGHpA

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

    Insert

    tTA2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Beta Globin minimal promoter

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-scFv-GCN4-sfGFP-GB1-dWPRE

    Plasmid

    #60907

    Purpose

    The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    scFv-GCN4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR_DKO

    Plasmid

    #183192

    Purpose

    Plasmid with Cas9, two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

    Depositor

    Lang Li

    Article

    Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, mU6

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Yes-EGFP

    Plasmid

    #110497

    Purpose

    Fluorescent Yes

    Depositor

    Bernardo Mainou

    Insert

    Yes (YES1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV9)

    Viral Prep

    #55645-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Promoter

    Syn

    Tags

    EYFP (Cre- and Flp-dependent)

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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