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Plasmid#103165PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-516b-5p
Plasmid#103601PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-516b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-516b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-ERR3 S57,81,219A
Plasmid#37850DepositorInsertEstrogen-related receptor gamma (Esrrg Mouse)
UseTagsHAExpressionMammalianMutationchanged Serines 57, 81, and 219 to Alanine (AGC t…PromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-RAB10 T23N-WPRE-UbC-Emerald
Plasmid#203802PurposeLentiviral vector plasmid expressing human RAB10 mutation T23N (dominant negative mutant) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationT23NPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-mApg5(K130R)
Plasmid#22959DepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseTagsEGFPExpressionMammalianMutation130 Lysine to ArgininePromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Del-6
Plasmid#14969DepositorInsertc-myc promoter (-109/+334) (MYC Human)
UseLuciferaseTagsExpressionMammalianMutation-109/+334 (relative to the P2 transcription initi…PromoterAvailable sinceMay 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa597-1407
Plasmid#19146DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 597-1407 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/Venus-mATG5
Plasmid#58739PurposeExpresses Venus-mATG5 in mammalian cellsDepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseRetroviralTagsVenusExpressionMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC4 (ARPC4 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT
Plasmid#140201PurposeFungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.DepositorTypeEmpty backboneUseCRISPR and Synthetic Biology; Cloning of crrna an…TagsdLbCas12a crRNA scaffoldExpressionMutationPromoterPgpdAAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-138-5p
Plasmid#103233PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-138-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-138-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-19b-3p
Plasmid#103327PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMYC BRGI aa1400-1648
Plasmid#19145DepositorInsertSWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
UseTagsMycExpressionMammalianMutationhuman BRG1 aa 1400-1648 (PCR product)PromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
ArpC5_pLib
Plasmid#173682PurposeArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC5 (ARPC5 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
ArpC1_pLib
Plasmid#173678PurposeArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC1 (ARPC1B Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-TN
Plasmid#86080PurposeA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged threonine 22 to asparginePromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-mApg5(K130R)
Plasmid#22957DepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseTagsExpressionMammalianMutation130 Lysine to ArgininePromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
CMV-REX-GECO0.9
Plasmid#61247PurposeExpresses REX-GECO0.9 in mammalian cellsDepositorInsertREX-GECO0.9
UseTagsExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterCMVAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7f-5p
Plasmid#103156PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7f-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7f-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-mATG5
Plasmid#58744PurposeExpresses /SECFP-ATG5 in mammalian cellsDepositorInsertautophagy related genes 5 (Atg5 Mouse)
UseRetroviralTagsSECFPExpressionMutationPromoterAvailable sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-133a-3p
Plasmid#103221PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-133a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-133a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-125b-5p
Plasmid#103191PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-125b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-125b-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-26a-5p
Plasmid#103379PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-26a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-26a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-101-5p
Plasmid#103166PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-101-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-101-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-7-1-3p
Plasmid#103722PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-7-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-7-1-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-16-5p
Plasmid#103272PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-16-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-16-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-103a-3p
Plasmid#103168PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-103a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-103a-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ArpC2_pLib
Plasmid#173679PurposeArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC2 (ARPC2 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only