We narrowed to 2,618 results for: ubiquitin

1. pOPINK-OTUD6B (full-length, aa 1-293)

   Plasmid

   #61418

   Purpose

   Expresses human OTUD6B (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6B (OTUD6B Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p7130 pHAGE-P-CMVt-N-HA-GAW-KIF20A

   Plasmid

   #100156

   Purpose

   Expresses KIF20A

   Depositor

   Peter Howley

   Article

   Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

   Insert

   KIF20A (KIF20A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

   Plasmid

   #63565

   Purpose

   Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysines at 5, 9, 118, 119, 125, 127, and 129 are…

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

4. His-SUMO-hDDI2-D252N_pET11a

   Plasmid

   #169122

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hDDI (DDI2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D252N

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOPINK-OTUD6A (full-length, aa 1-288)

   Plasmid

   #61416

   Purpose

   Expresses human OTUD6A (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD6A (OTUD6A Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV6-AN-DDK_WT POLI

   Plasmid

   #131228

   Purpose

   Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   DNA polymerase iota (POLI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Coding sequence has been codon optimised for expr…

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-N-Myc_775-1102 USP7

   Plasmid

   #131250

   Purpose

   Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCS4 3XFLAG-PPARgamma2

   Plasmid

   #78770

   Purpose

   To overexpress PPARgamma2 in Mammalian Cells

   Depositor

   Jaewhan Song

   Article

   Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

   Insert

   PPAR_2 (Ppargc1a Mouse)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pETDUET-GST AO7REwt

   Plasmid

   #213935

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p7137 pHAGE-P-CMVt-N-HA-GAW-EIF3M

    Plasmid

    #100149

    Purpose

    Expresses EIF3M

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    EIF3M (EIF3M Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p7129 pHAGE-P-CMVt-N-HA-GAW-HM13

    Plasmid

    #100155

    Purpose

    Expresses HM13

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    NM_030789.3 (HM13 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-RNF51

    Plasmid

    #126546

    Purpose

    Expresses GFP-tagged RNF51 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

    Insert

    RNF51 (BMI1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX4T1-ISG15 C78SN13Y

    Plasmid

    #165106

    Purpose

    bacterial expressio of a GST fusion of Human ISG15 C78SN13Y

    Depositor

    Arthur Haas

    Article

    Narasimhan et al J Biol Chem. 2005 Jul 22. 280(29):27356-

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    C78SN13Y; The sequence of ISG15-C78S/N13Y contai…

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastbac1-GST-hUSP7

    Plasmid

    #63573

    Purpose

    Expression of synthetic human USP7 tagged with GST

    Depositor

    Titia Sixma

    Article

    Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

    Insert

    USP7 (USP7 Human, Synthetic)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Synthetic based on human protein sequence (please…

    Promoter

    Polyhedrin

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT-Flag-Cul5

    Plasmid

    #31984

    Purpose

    Mammalian expression of Flag-tagged Cul5

    Depositor

    Joan Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    Cul5 (CUL5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCMV10-Flag-Fbxl21

    Plasmid

    #48752

    Purpose

    Expression of FLAG-tagged Fbxl21

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Fbxl21 (Fbxl21 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1 UBXN2A

    Plasmid

    #113505

    Purpose

    Bacterial expression of GST-tagged UBXN2A

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    UBXN2A (UBXN2A Human)
    

    Use

    Tags

    GST-Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. His-SUMO- flag-hDDI2-D252N_pET11a

    Plasmid

    #169123

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D252N

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge