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Showing: 121 - 140 of 67199 results

  3. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71667-plasmid-map-sequence-id-243369

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  5. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Publication
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    155297-plasmid-map-sequence-id-307858

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  8. pdCas9-DNMT3A-PuroR_v2

    Plasmid
    #74407
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74407-plasmid-map-sequence-id-140317

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  9. S. cerevisiae Advanced Gateway Destination Vectors

    Plasmid kit
    #1000000011
    Purpose
    Destination vectors for tagging and expression of genes in S. cerevisiae.
    Depositor
    Susan Lindquist
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Yeast Expression
    Cloning type
    Gateway
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  11. pEGFP-N1 human cofilin S3A

    Plasmid
    #50860
    Purpose
    expresses constituvely active cofilin fused to EGFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 S3A (CFL1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S3A constitutively active
    Promoter
    CMV
    Available since
    Feb. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50860-plasmid-map-sequence-id-133100

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  12. pdCas9-DNMT3A-EGFP (ANV)

    Plasmid
    #71685
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71685-plasmid-map-sequence-id-322543

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  13. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Publication
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158762-plasmid-map-sequence-id-309495

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  14. pEGFP-N1 human cofilin S3E

    Plasmid
    #50861
    Purpose
    expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells
    Depositor
    James Bamburg
    Publication
    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cofilin 1 S3E (CFL1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S3E psuedo-phosporylated, non-activatable
    Promoter
    CMV
    Available since
    Feb. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50861-plasmid-map-sequence-id-133102

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  16. MoClo Yeast Toolkit

    Plasmid kit
    #1000000061
    Purpose
    Collection of standardized parts for hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression.
    Depositor
    John Dueber
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. M-ST1-sgRNA

    Plasmid
    #48672
    Purpose
    Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG
    Depositor
    George Church
    Publication
    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter
    Use
    CRISPR
    Promoter
    hU
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    48672-plasmid-map-sequence-id-71846

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Showing: 121 - 140 of 67199 results