Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 56,262 results for: plasmid

Showing: 121 - 140 of 56262 results

  1. fru-T2A-QF2 HDR plasmid

    Plasmid
    #141099
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    Use
    Donor template
    Available Since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141099-plasmid-map-sequence-id-292289

    Enlarge

  2. Equalizer-M plasmid with onboard mCherry cassette

    Plasmid
    #169734
    Purpose
    Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169734-plasmid-map-sequence-id-334141

    Enlarge

  3. Equalizer-L plasmid with onboard mCherry cassette

    Plasmid
    #169735
    Purpose
    Equalizer-L plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169735-plasmid-map-sequence-id-334142

    Enlarge

  4. Ir7f-T2A-QF2 HDR plasmid

    Plasmid
    #140942
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7f gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7f-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7f-right-HDR-arm
    Mutation
    4 point mutations (annotated on plasmid map) were…
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140942-plasmid-map-sequence-id-313600

    Enlarge

  5. Ir7a-T2A-QF2 HDR plasmid

    Plasmid
    #140943
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140943-plasmid-map-sequence-id-313597

    Enlarge

  11. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169737
    Purpose
    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169737-plasmid-map-sequence-id-334143

    Enlarge

  12. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

    Enlarge

  13. pLK89 - Edit-directing plasmid cloning insert

    Plasmid
    #98846
    Purpose
    Contains the insert used for step 2 of edit-directed plasmid cloning
    Depositor
    Leonid Kruglyak
    Insert
    KanR
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Changed sequence to remove a MluI cut site
    Available Since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    98846-plasmid-map-sequence-id-189230

    Enlarge

Showing: 121 - 140 of 56262 results