We narrowed to 747 results for: streptococcus -(crispr) -(cas9)

1. BB3cH_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104907

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-T3-st1CAS9-pA

   Plasmid

   #131542

   Purpose

   Expresses S. thermophilus 1-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2016 Jun 16.

   Insert

   Codon optimized st1Cas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster vasa

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB[nos-Cas9 PUb-ZsGreen]

   Plasmid

   #169011

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster nanos

   Available Since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Sox1/2/3-2373/+3>nls::Cas9::nls

   Plasmid

   #59989

   Purpose

   Sox1/2/3 (SoxB1) driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Sox1/2/3 (SoxB1) -2373/+3

   Available Since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pspCas9-3'UTR-Tetra-com-vector

   Plasmid

   #132553

   Purpose

   Expresses spCas9 and sgRNA scaffold with com aptamer replacing the Tetraloop

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Inserts

   Cas9
   sgRNA scaffold with com replacing the Tetraloop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104910

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104912

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104908

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid

    #104906

    Purpose

    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHS23_10xHis_MBP_TEV_SpG-Cas9

    Plasmid

    #244826

    Purpose

    Bacterial expression of SpG Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpG Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS24_10xHis_MBP_TEV_SpRY-Cas9

    Plasmid

    #244827

    Purpose

    Bacterial expression of SpRY Cas9 for protein purification

    Depositor

    Jennifer Doudna

    Article

    Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

    Insert

    SpRY Cas9
    

    Use

    CRISPR

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…

    Promoter

    T7

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-KRAB

    Plasmid

    #191314

    Purpose

    piggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    KRAB-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. AW01_pU.CAG.eSpCas9(1.1).rBGpA

    Plasmid

    #199255

    Purpose

    Expression construct encoding the high specificity eSpCas9(1.1) nuclease

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

    Insert

    High specificity eSpCas9(1.1) nuclease
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    K848A K1003A R1060A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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