We narrowed to 4,471 results for: ara-2

1. pBG-ttLbUV2

   Plasmid

   #231386

   Purpose

   LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   ttLbCas12a Ultra V2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D156R and E795L

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-synP-FLEX-splitTVA-EGFP-B19G

   Plasmid

   #52473

   Purpose

   Expresses splitTVA-EGFP-B19G

   Depositor

   Ian Wickersham

   Article

   Kohara et al Nat Neurosci. 2014 Feb;17(2):269-79. doi

   Has Service

   AAV1

   Insert

   TVA-P2A-EGFP-P2A-B19G
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin-1

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-7aa-ActA-pEGFP-C1

   Plasmid

   #177406

   Purpose

   To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

   Depositor

   David Andrews

   Article

   Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

   Insert

   ActA tail anchor (actA Listeria monocytogenes)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUBQ10:AtFLS2

   Plasmid

   #202186

   Purpose

   Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

   Depositor

   Gitta Coaker

   Article

   Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

   Insert

   Flagellin sensing 2 (FLS2 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Tags

   Expression

   Mutation

   7mer insertion in the AAV2 cap

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 HRPT2 136X

   Plasmid

   #11049

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   HRPT2 136X (CDC73 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncation mutant with a stop codon introduced at…

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 HRPT2 227X

   Plasmid

   #11050

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   HRPT2 227X (CDC73 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncation mutant with a stop codon introduced at…

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-His8-aCat(660-906)

   Plasmid

   #203333

   Purpose

   Bacterial expression of alpha-catenin F-actin binding domain

   Depositor

   Tina Izard

   Article

   Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   8xHis with precision protease cleavage site

   Expression

   Bacterial

   Mutation

   aa 660-906 only

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-His8-aCat(660-865)

    Plasmid

    #203335

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-865 only

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-His8-aCat(660-885)

    Plasmid

    #203334

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Use

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-885 only

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. S1030

    Bacterial Strain

    #105063

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Carlson et al Nat Chem Biol. 2014 Mar;10(3):216-22. do

    Bacterial Resistance

    Streptomycin and tetracycline

    Available since

    March 21, 2018

    Availability

    Academic Institutions and Nonprofits only

13. pROS13

    Plasmid

    #107927

    Purpose

    kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11-3’_8xN_PAM-site1 (RTW554)

    Pooled Library

    #160132

    Purpose

    Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Species

    Synthetic

    Use

    CRISPR

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

15. p11-5’_10xN_PAM-site3 (RTW572)

    Pooled Library

    #160134

    Purpose

    Plasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Species

    Synthetic

    Use

    CRISPR

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

16. p11-5’_10xN_PAM-site4 (RTW574)

    Pooled Library

    #160135

    Purpose

    Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Species

    Synthetic

    Use

    CRISPR

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

17. DNMT3ACD-CRY2-EGFP

    Plasmid

    #82556

    Purpose

    Encodes DMNT3 under LITE2.0 system

    Depositor

    Joseph Irudayaraj

    Article

    Lo et al Sci Rep. 2017 Feb 9;7:42047. doi: 10.103

    Inserts

    DNMT3A (DNMT3A Human)
    CRY2 (CRY2 Mustard Weed)
    EGFP
    

    Use

    AAV

    Tags

    2A, NLS(alpha-imp), and NLS-VP64

    Expression

    Mammalian

    Mutation

    CRY2PHR (N-terminal photolyase homology region do…

    Promoter

    EF-1alpha

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-Puro-ARHGAP26

    Plasmid

    #69467

    Purpose

    Expresses ARHGAP26

    Depositor

    Axel Hillmer

    Article

    Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

    Insert

    ARHGAP26 (ARHGAP26 Human)
    

    Use

    Retroviral

    Tags

    HA TAG

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15b-At 14-3-3 nu

    Plasmid

    #44940

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 nu (GRF7 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pET15b-At 14-3-3 epsilon

    Plasmid

    #44936

    Depositor

    Robert Ferl

    Article

    Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

    Insert

    14-3-3 epsilon (GRF10 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only