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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,217 results for: sgRNA

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Showing: 1381 - 1400 of 9217 results

  1. (pRZ219) AAV: U6-gRNA-EF1a-mScarlet

    Plasmid
    #254585
    Purpose
    AAV backbone expressing a U6-driven sgRNA with SapI spacer and mScarlet from the EF1a promoter
    Depositor
    Jesse Engreitz
    Insert
    sgRNA with SapI spacer
    Use
    AAV
    Available since
    May 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    254585-plasmid-map-sequence-id-513279

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  2. PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP

    Plasmid
    #172669
    Purpose
    For expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition site
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNF2 pegRNA and RNF2_nick-sgRNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    172669-plasmid-map-sequence-id-339966

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  3. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid
    #129531
    Purpose
    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR
    Depositor
    Erik Sontheimer
    Publication
    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d
    Inserts
    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    Use
    AAV
    Tags
    FLAG/NLS
    Promoter
    U6 and cytomegalovirus-enhancer chicken β-actin p…
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129531-plasmid-map-sequence-id-255632

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  5. pAAV- GfaABC1D::Cas9-HA-sgRNA-Non-Targeting

    Plasmid
    #241026
    Purpose
    GfaABC1D promoter driven HA-tagged SaCas9 together with U6 driven expression of a non-targeting sgRNA; used as control for SaCas9 based knock-outs in murine astrocytes
    Depositor
    Mark Verheijen
    Publication
    Badia-Soteras et al Biol Psychiatry. 2023 Aug 1;94(3):226-23
    Insert
    U6 driven empty sgRNA
    Use
    AAV and CRISPR
    Tags
    SaCas9 + 3X HA-Tag
    Expression
    Mammalian
    Promoter
    GfaABC1D
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    241026-plasmid-map-sequence-id-482053

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  6. pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126698
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting human SCGB3A2 locus
    Expression
    Mammalian
    Promoter
    Human U6
    Available since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126698-plasmid-map-sequence-id-245766

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  8. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-targeting sgRNA human (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    71830-plasmid-map-sequence-id-131025

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  10. pColE1_sgRNA_M4NS_AmpR

    Plasmid
    #119156
    Purpose
    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with four mismatches in the non-seed region
    Depositor
    Vincent Noireaux
    Publication
    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with four mismatches in the non-seed region
    Use
    Crispr
    Promoter
    J23119
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    119156-plasmid-map-sequence-id-229445

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  11. pColE1_sgRNA_M1NS_AmpR

    Plasmid
    #119155
    Purpose
    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR with one mismatch in the non-seed region
    Depositor
    Vincent Noireaux
    Publication
    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR, with one mismatch in the non-seed region
    Use
    Crispr
    Promoter
    J23119
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    119155-plasmid-map-sequence-id-229443

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  12. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid
    #98975
    Purpose
    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.
    Depositor
    Jan Karlseder
    Publication
    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LMNA sgRNAs pair 4 and Cas9(D10A)
    Expression
    Mammalian
    Available since
    Nov. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    98975-plasmid-map-sequence-id-199218

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Showing: 1381 - 1400 of 9217 results