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Plasmid#68119PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. ElegansDepositorInsertrab-3::NLS::GCaMP6s
ExpressionWormPromoterrab-3Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHCMV-VSVgSS-pAG-TM
Plasmid#207318PurposeFunctional incorporation of protein AG (pAG) fusion protein into envelope of virionsDepositorInsertprotein AG
ExpressionMammalianPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP1339
Plasmid#119271Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SspBmicro-mCh-p60
Plasmid#190168PurposeExpresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSspBmicro-mCherry-p60
TagsSspBmicro-mCherryExpressionMammalianAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-GINKO2
Plasmid#177116PurposeBacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO2DepositorInsertGINKO2
Tags6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…ExpressionBacterialAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EF1a_ASCL1_P2A_Hygro_Barcode
Plasmid#120427PurposeBarcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Smoothened1-N-18
Plasmid#55134PurposeLocalization: Cilium, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSmoothened1
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ909
Plasmid#44480DepositorInsertMinimal Mos1, unc-119, MCS
UseMultiple cloning site vectorAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-Ubiquitin-K11R
Plasmid#121154PurposeMammalian expression of HA tagged ubiquitin K11RDepositorAvailable sinceFeb. 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEmerald-Ezrin-N-14
Plasmid#54090PurposeLocalization: Focal Adhesions/Membrane, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEzrin (EZR Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-4xTR-TUBE
Plasmid#110312PurposeE. coli expression and purification of 4xTR-TUBEDepositorInsert4xTR-TUBE (UBQLN1 Human)
TagsN-terminal His6-T7 tagExpressionBacterialMutationAll Arg residues in the UBA domain are mutated to…PromoterT7Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0101
Plasmid#90998PurposeModule A, Promoter: 35S, Gene: AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAtCas9
UseCRISPRPromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCYC1m_yeGFP
Plasmid#64389Purposeencodes yeast enhanced GFP under control of minimal pCYC1 promoterDepositorPublicationInsertyeast enhanced GFP
ExpressionYeastPromoterpCYC1Available sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJ404-DogCatcher-sfGFP
Plasmid#171930PurposeExpress DogCatcher-superfolder GFP protein in bacterial cytoplasmDepositorInsertDogCatcher-sfGFP
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT5Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bfloGFPa1
Plasmid#60749PurposeBacterial expression of Amphioxus GFPa1DepositorInsertGFPa1
Tags6xHisExpressionBacterialPromoterT7Available sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry-UBQ
Plasmid#78932PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-EB3-7
Plasmid#55037PurposeLocalization: MT End Binding Protein, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3 (MAPRE3 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SpySwitch
Plasmid#184225PurposeExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.DepositorInsertSpySwitch
TagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by