Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,294 results for: otos

Clear keywords
Showing: 2101 - 2130 of 10294 results

  2. CLYBL-TO-hNIL-BSD-mApple

    Plasmid
    #124230
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2-ISL1-LHX3 for differentiation of iPSCs into lower motor neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Insert
    NGN2, ISL1, LHX3 (NEUROG2, ISL1, LHX3 Human)
    Expression
    Mammalian
    Available Since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    124230-plasmid-map-sequence-id-240604

    Enlarge

  5. pUCIDT-attL1-Worm ABeta-attR5

    Plasmid
    #160437
    Purpose
    Entry vector for cloning nematode-codon-optimized Amyloid Beta(1-42) using 2-fragment gateway recombination
    Depositor
    Nicholas Tolwinski
    Article
    Kaur et al Bio Protoc. 2020 Dec 5;10(23):e3856. doi
    Insert
    Homo sapiens amyloid beta precursor protein (APP Human, Nematode)
    Tags
    Cleavage signal
    Expression
    Bacterial
    Mutation
    Codon-optimized for expression in C. elegans
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    160437-plasmid-map-sequence-id-314980

    Enlarge

  8. pAAV-Ef1a-DIO-PPO-Venus

    Plasmid
    #139505
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV8 and AAV9
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Promoter
    Ef1a
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139505-plasmid-map-sequence-id-276175

    Enlarge

  12. pAAV-CAG-DIO-PPO-Venus

    Plasmid
    #139504
    Purpose
    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
    Depositor
    Michael Bruchas
    Article
    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11
    Has Service
    AAV5 and AAV9
    Insert
    parapinopsin
    Use
    AAV
    Tags
    Venus tag following 3x Ala linker
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    139504-plasmid-map-sequence-id-276180

    Enlarge

  13. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

    Enlarge

  14. pTK41

    Plasmid
    #236110
    Purpose
    Expresses the motor domain of rat KIF5C fused with SNAP tag and ALFA tag nanobody
    Depositor
    Shinsuke Niwa
    Article
    Kita et al Cell Rep Phys Sci, 6 (2): 102440. 19 Feb
    Inserts
    KIF5C(C7S)(1-430)-SNAP-His (Kif5c Rat)
    KIF5C(C7S)(1-430)-ALFA tag nanobody-StrepII tag (Kif5c Rat)
    Tags
    ALFA tag nanobody, His tag, SNAP tag, and StrepII…
    Expression
    Bacterial
    Mutation
    C7S mutation is introduced. 1-430 aa is encoded.
    Promoter
    T7
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    236110-plasmid-map-sequence-id-472830

    Enlarge

  17. pSems-leader-mXFPm-IFNGR1(18-489)

    Plasmid
    #192786
    Purpose
    Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR1 (IFNGR1 Human)
    Tags
    Ig k-chain leader sequence and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192786-plasmid-map-sequence-id-379664

    Enlarge

  18. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

    Plasmid
    #132393
    Purpose
    Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site
    Depositor
    Gerold Schmitt-Ulms
    Article
    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:
    Insert
    human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
    Use
    CRISPR and Cre/Lox
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Proline 301 to Leucine (P301L)
    Promoter
    TREtight
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    132393-plasmid-map-sequence-id-341619

    Enlarge

  19. pSems-leader-mXFPe-IFNGR2(30-337)

    Plasmid
    #192787
    Purpose
    Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNGR2 (IFNGR2 Human)
    Tags
    Ig k-chain leader sequence and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192787-plasmid-map-sequence-id-379660

    Enlarge

  22. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Article
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Human, Avena sativa (oat))
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

    Enlarge

  24. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

    Enlarge

  26. pSems-leader-SNAPf-mXFPe-IFNAR1(28-557)

    Plasmid
    #192785
    Purpose
    Expression of SNAPf and mXFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPe
    Expression
    Mammalian
    Mutation
    mXFPe: tyrosine 66 to phenylalanine, asparagine 1…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192785-plasmid-map-sequence-id-379661

    Enlarge

  27. pAAV-hsynapsin-DIO-adra2a-shRNA-mCherry

    Plasmid
    #245063
    Purpose
    AAV-transgene knocking down adra2a receptor (adrenergic 2a receptor) transcripts cell type-selectively. Using the pPRIME. system, this generates mir30-derived shRNAs and a marker from the same RNA.
    Depositor
    William Wisden
    Article
    Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12
    Insert
    AAV-human synapsin promoter-DIO-adra2a-shRNA-mCherry (Adra2a Mouse, Synthetic)
    Use
    AAV, Cre/Lox, and RNAi
    Promoter
    human synapsin
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    245063-plasmid-map-sequence-id-489355

    Enlarge

Showing: 2101 - 2130 of 10294 results