We narrowed to 6,134 results for: nls

1. 6xHis-MBP-huMb3Cas12a

   Plasmid

   #174677

   Purpose

   Expresses 6xHis-MBP tagged Mb3Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb3Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMS2-YFP

   Plasmid

   #27122

   Depositor

   Robert Singer

   Article

   Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

   Insert

   MS2
   

   Use

   Tags

   EYFP, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLSLGFP

   Plasmid

   #51494

   Purpose

   pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GFP
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Expression

   Mutation

   Promoter

   virion-sense LIR and 2x35S

   Available since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Cry2FL-VP64

   Plasmid

   #60554

   Purpose

   Expresses Cry2FL-VP64 in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   Cry2FL-VP64
   

   Use

   Tags

   FLAG, HA tag, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. vHB16

   Plasmid

   #193455

   Purpose

   Expression of GFP-Cas9-NLS and gRNA in A. castellanii

   Depositor

   Chantal Abergel

   Article

   Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

   Insert

   tRNA-gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRETightBI-RY-0

   Plasmid

   #31463

   Depositor

   Phil Sharp

   Article

   Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

   Insert

   eYFP and mCherry
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ11438

   Plasmid

   #183204

   Purpose

   pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

   Depositor

   Stanley Qi

   Article

   Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

   Insert

   hyperdCas12 base editor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MS2-Snapf

    Plasmid

    #65860

    Purpose

    Expresses RNA-binding protein MS2 with fast SNAP fusion tag

    Depositor

    Aaron Hoskins

    Article

    Carrocci et al Analyst. 2014 Jan 7;139(1):44-7. doi: 10

    Inserts

    MS2
    Fast SNAP
    

    Use

    Tags

    HA and NLS

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pLEXY (pDN137)

    Plasmid

    #72656

    Purpose

    golden gate entry vector for mCherry-LEXY tagging of proteins (no constitutive NLS)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Type

    Empty backbone

    Use

    Golden gate entry vector

    Tags

    mCherry-LEXY

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 6xHis-MBP-huBoCas12a

    Plasmid

    #174673

    Purpose

    Expresses 6xHis-MBP tagged BoCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBoCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 6xHis-MBP-huPb2Cas12a

    Plasmid

    #174686

    Purpose

    Expresses 6xHis-MBP tagged Pb2Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPb2Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ11440

    Plasmid

    #183203

    Purpose

    pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    hyperdCas12 base editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKF295

    Plasmid

    #72871

    Purpose

    expression of improved FLP recombinase

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    FLP recombinase (flp Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    P2->S, added NLS

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNSEN-d2

    Plasmid

    #59763

    Purpose

    Expresses nuclear EGFP and Tomato in mammalian cells

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2009 Jul 30;460(7255):642-6. doi

    Inserts

    d2-EGFP
    Tomato
    

    Use

    Tags

    3X NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and SV40 promoter

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

    Plasmid

    #62345

    Purpose

    Expresses MCP-tdiRFP670 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    MCP-tdiRFP670
    

    Use

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCNPpo6

    Plasmid

    #46086

    Depositor

    Raymond Monnat

    Article

    Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

    Insert

    PpoI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EZ-L545

    Plasmid

    #173024

    Purpose

    Encodes OptoEXP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOG231

    Plasmid

    #17736

    Depositor

    Geoff Wahl

    Article

    O'Gorman et al Proc Natl Acad Sci U S A. 1997 Dec 23;94

    Insert

    NLS-Cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

22. EZ-L580

    Plasmid

    #173023

    Purpose

    Encodes OptoAMP4 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNuc-TrxRFP1

    Plasmid

    #98998

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and nuclear localization sequence
    

    Use

    Tags

    His-tag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCNCre3

    Plasmid

    #46088

    Depositor

    Raymond Monnat

    Article

    Monnat RJ et al Biochem Biophys Res Commun. 1999 Feb 5;2

    Insert

    CreI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMXS-IRES-BLAST co hPHGDH

    Plasmid

    #154915

    Purpose

    Express codon optimized human PHGDH

    Depositor

    Richard Possemato

    Article

    Richard et al Richard Possemato Lab plasmids

    Insert

    PHGDH, codon optimized (PHGDH Human, Synthetic)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for expression in human cells

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKI1.1

    Plasmid

    #85807

    Purpose

    CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    May 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBSKΔB-TRE-dCas9-EGFP

    Plasmid

    #62347

    Purpose

    Plasmid for tet-inducible expression of dCas9-EGFP in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

    Insert

    dCas9-EGFP
    

    Use

    CRISPR

    Tags

    HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRETightBI-RY-HMGA2wt

    Plasmid

    #31469

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pIR190

    Plasmid

    #64812

    Purpose

    TCP4 expression in yeast

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al PLoS Genet. 2013 Mar;9(3):e1003374. doi:

    Insert

    TCP4 (TCP4 Mustard Weed)
    

    Use

    Tags

    GAL4 Activation Domain and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNXRVj-HACre

    Plasmid

    #78407

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    aur1R (aur1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Gly 240 to Cys

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BCJJ180

    Plasmid

    #117516

    Purpose

    Cas9_4 Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_4:GCTT:BsaI
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. RHD_exon1_TALEN_R

    Plasmid

    #62240

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Right
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. RHD_exon1_TALEN_L

    Plasmid

    #62239

    Purpose

    expression of TALEN targeting human RHD exon1

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon1_TALEN_Left
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTRETightBI-RY-4

    Plasmid

    #31465

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pTRETightBI-RY-1

    Plasmid

    #31464

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

37. 6xHis-MBP-huLb5Cas12a

    Plasmid

    #174684

    Purpose

    Expresses 6xHis-MBP tagged Lb5Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huLb5Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTSK368

    Plasmid

    #34551

    Depositor

    James McNally

    Article

    Karpova et al Science. 2008 Jan 25;319(5862):466-9.

    Insert

    MS2-CP-NLS
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Met25

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRETightBI-RY-7

    Plasmid

    #31466

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pTRETightBI-RY-1pf

    Plasmid

    #31467

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

41. pTRETightBI-RY-HMGA2mutant

    Plasmid

    #31470

    Depositor

    Phil Sharp

    Article

    Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

    Insert

    eYFP and mCherry
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

42. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pYZ675

    Plasmid

    #132954

    Purpose

    crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a Schizosaccharomyces pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

    Insert

    dFnCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    adh1

    Available since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. 6xHis-MBP-huPcCas12a

    Plasmid

    #174681

    Purpose

    Expresses 6xHis-MBP tagged PcCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPcCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 6xHis-MBP-huCMtCas12a

    Plasmid

    #174680

    Purpose

    Expresses 6xHis-MBP tagged CMtCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMtCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. 6xHis-MBP-huPdCas12a

    Plasmid

    #174682

    Purpose

    Expresses 6xHis-MBP tagged PdCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPdCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pHdCas9-mammalian

    Plasmid

    #138415

    Purpose

    Expression of Himar-dCas9 in mammalian cells

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Use

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. RHD_exon4_TALEN_L

    Plasmid

    #62241

    Purpose

    expression of TALEN targeting human RHD exon4

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon4_TALEN_Left
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. RHD_exon4_TALEN_R

    Plasmid

    #62242

    Purpose

    expression of TALEN targeting human RHD exon4

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

    Insert

    human RHD_exon4_TALEN_Right
    

    Use

    TALEN

    Tags

    HA tag, NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. p35ST

    Plasmid

    #51514

    Purpose

    pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    T30 TALEN pair
    us:NPTII repair template
    

    Use

    Plant expression t-dna

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    2x35S

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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