We narrowed to 2,574 results for: PORA

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-H2BmCherry-OptoJNKi

Plasmid

#89741

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Optogenetics

Tags

Histone2B and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

Plasmid

#154779

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Tags

LaG17

Expression

Mammalian

Mutation

204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA_CDKN1A

Plasmid

#136523

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

CDKN1A (CDKN1A Human)

Use

Entry vector for gateway cloning

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500

Plasmid

#92316

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pSA143 Cas9-BFP with core cHS4 insulators

Plasmid

#249153

Purpose

Overexpression of SpCas9-BFP and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

Cas9-TagBFP with core cHS4 insulators

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD231 PPH-T2A-dCas9-NFZ-P2A-BFP

Plasmid

#249155

Purpose

Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PPH-T2A-dCas9-NFZ-P2A-mTagBFP2

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-TRE>hHES3:T2A:EGFP

Plasmid

#236630

Purpose

Hygromycin selected lentiviral construct with tetracyclin-inducible human HES3

Depositor

Genevieve Kendall

Article

Kent et al Sci Rep. 2024 Sep 5;14(1):20773. doi: 10

Inserts

HES3 (HES3 Human)
EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized based on a variant of wildtype GF…

Promoter

TRE

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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FUGW-RUSH-LAMP1-mScarleti

Plasmid

#228526

Purpose

Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells.

Depositor

Ginny Farias

Article

Li et al Nat Commun. 2024 Dec 30;15(1):10829. doi

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP), Streptavidin-…

Expression

Mammalian

Promoter

Ubc

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR137

Plasmid

#226809

Purpose

Gateway entry vector for GPR137

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TAPP1(PHD)

Plasmid

#221757

Purpose

Stable expression of GFP-TAPP1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLEKHA1 (PLEKHA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TAPP1 (PHD) (amino acids 184-304)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-Btk1(PHD)-GFP

Plasmid

#221756

Purpose

Stable expression of Btk1(PHD)-GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

Btk (BTK Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–177

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pSUMO-β′-COPI-WD40-Tyr33Ala

Plasmid

#208170

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Tyr33Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Tyr33Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Glu

Plasmid

#218461

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Glu substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Glu substitution (S )

Expression

Mammalian

Mutation

Thr1273AGlu substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SSH-pcDNA3.1-SSH-SARS-CoV-2-S-Thr1273Asp

Plasmid

#218462

Purpose

Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Thr1273Asp substitution)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

SARS-CoV-2 spike protein (1273 residues) carrying Thr1273Asp substitution (S )

Expression

Mammalian

Mutation

Thr1273Asp substitution

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-T394A

Plasmid

#204994

Purpose

Expresses shRNA resistant GFP-RepoMan T394A Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell devision cycle associated 2 (CDCA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Threonine 394 to Alanin

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-RepoMan-RATA

Plasmid

#184270

Purpose

Expresses shRNA resistant GFP-RepoMan RATA Mutant

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

cell division cycle associated 2 (CDCA2 Human)

Tags

3xHA and GFP

Expression

Mammalian

Mutation

V383A, F395A

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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pROS_phleo-Tps1/Gsy2

Plasmid

#196612

Purpose

Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

TPS1, GSY2 (TPS1, GSY2 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pROS13-Tps2/Gsy1

Plasmid

#196613

Purpose

Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

S000001911 (TPS2, GSY1 Budding Yeast)

Expression

Yeast

Promoter

SNR52

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pUGP1.1

Plasmid

#196615

Purpose

Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)

Tags

AID(71-114) and mCherry

Expression

Yeast

Promoter

Native promoter

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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