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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,545 results for: Tef;

Showing: 1461 - 1480 of 1545 results

  2. FRT_htr2c(c)-GFP

    Plasmid
    #79677
    Purpose
    serotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFP
    Depositor
    Stefan Stamm
    Article
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Insert
    serotonin receptor 2C (HTR2C Human)
    Use
    Tags
    EGFP in Frame
    Expression
    Mammalian
    Mutation
    splice site of exon Vb in consensus
    Promoter
    CMV
    Available since
    Aug. 30, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pDIV313

    Plasmid
    #204956
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger
    Depositor
    Uffe Mortensen
    Article
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Aspergillus fumigatus U3 promoter, Aspergillus ni…
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    204956-plasmid-map-sequence-id-415598

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  5. pMH-SFB-USP7

    Plasmid
    #99393
    Purpose
    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7
    Depositor
    Michael Huen
    Article
    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Insert
    USP7 (USP7 Human)
    Use
    Tags
    SFB (S protein-FLAG-Streptavidin binding peptide)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99393-plasmid-map-sequence-id-190612

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  10. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Use
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Use
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  12. pcDNA3.1_ZKSCAN1_Tau7-12WT

    Plasmid
    #194166
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 7,9-12. Expresses the tau circRNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 7-12 WT
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194166-plasmid-map-sequence-id-381821

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  13. pcDNA3.1_ZKSCAN1_Tau10-12WT

    Plasmid
    #194163
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by the wild-type tau cDNA exons 10-12. Expresses the tau circRNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT ZKSCAN1 intronic alu elements
    Use
    Tags
    3X flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194163-plasmid-map-sequence-id-381804

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  16. pcDNA3.1_Tau Authentic Alu 10-12WT

    Plasmid
    #194167
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 10-12 . Expresses the tau circular RNA 12-->10 WT with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 WT
    Use
    Tags
    3X Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194167-plasmid-map-sequence-id-381798

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  17. pcDNA3.1_Tau Authentic Alu 7-12WT

    Plasmid
    #194170
    Purpose
    Insert contains intronic Tau authentic Alu and repeat elements. The introns are flanked by the Wild Type tau cDNA exons 7,9-12 . Expresses the tau circular RNA 12-->7 WT with 3X flag tag in exon 7.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    microtubule-associated protein tau 7-12 WT
    Use
    Tags
    3x Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194170-plasmid-map-sequence-id-381818

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  18. pcDNA3.1_ZKSCAN1_Tau10-12V337M

    Plasmid
    #194165
    Purpose
    Insert contains intronic ZKSCAN1 Alu repeat elements flanked by tau cDNA exons 10-12 with FTLD-Tau V337M mutation. Expresses the tau circRNA 12-->10 V337M with 3X flag tag in exon 10.
    Depositor
    Stefan Stamm
    Article
    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129
    Insert
    Microtubule-associated protein tau 10-12 V337M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Valine 337 to Methinonine
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    194165-plasmid-map-sequence-id-381806

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Showing: 1461 - 1480 of 1545 results