Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 3,209 results for: EED

Clear keywords
Showing: 2961 - 3000 of 3209 results

  1. pYPQ265N2

    Plasmid
    #174001
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-UNG
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174001-plasmid-map-sequence-id-343426

    Enlarge

  2. pYPQ265O2

    Plasmid
    #174002
    Purpose
    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174002-plasmid-map-sequence-id-343429

    Enlarge

  3. pYPQ265L2

    Plasmid
    #174000
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174000-plasmid-map-sequence-id-343423

    Enlarge

  7. pMRBad-Z-C-DiB-RM

    Plasmid
    #168477
    Purpose
    C-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    Leucine Zipper + C-fragment of DiB-RM
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168477-plasmid-map-sequence-id-332736

    Enlarge

  8. pET11a-Z-N-DiB-RM

    Plasmid
    #168475
    Purpose
    N-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip protein
    Depositor
    Jens Meiler
    Article
    Bozhanova et al PLoS Comput Biol. 2021 Nov 8;17(11):e100
    Insert
    N-fragment of DiB-RM + Leucine Zipper
    Tags
    His-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    168475-plasmid-map-sequence-id-332732

    Enlarge

  9. pRWJB006

    Plasmid
    #160113
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under 35S promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    35S
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160113-plasmid-map-sequence-id-325470

    Enlarge

  10. pRWJB004

    Plasmid
    #160112
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial and Plant
    Promoter
    UBQ10
    Available Since
    Feb. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160112-plasmid-map-sequence-id-325469

    Enlarge

  11. pVH009

    Plasmid
    #80399
    Purpose
    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain and N-terminal 3xFLAG tag, …
    Expression
    Bacterial
    Available Since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80399-plasmid-map-sequence-id-155900

    Enlarge

  15. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

    Enlarge

  17. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162468-plasmid-map-sequence-id-350748

    Enlarge

  21. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

    Enlarge

  22. pPPEDs

    Plasmid
    #162469
    Purpose
    protoplast test vector of pPPED
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Use
    Prime editing destination vector for transient ex…
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine
    Available Since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162469-plasmid-map-sequence-id-350377

    Enlarge

  27. mutpRL-TK 3xUTR

    Plasmid
    #40758
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Article
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    3 copies of the CXCR4 small RNA target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  38. pMDS1

    Plasmid
    #239460
    Purpose
    For simultaneous monitoring of both transcription and translation of genes in plant tissues: C-terminus fusion
    Depositor
    Miguel de Lucas
    Article
    Banda et al Sci. Adv., 11, eadw9153 (2025)
    Type
    Empty backbone
    Tags
    VENUS:3xHA:2A:mTurqN7
    Expression
    Bacterial and Plant
    Available Since
    Oct. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    239460-plasmid-map-sequence-id-488473

    Enlarge

  39. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Tags
    His tag
    Expression
    Mammalian
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

    Enlarge

Showing: 2961 - 3000 of 3209 results