Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 116,638 results for: mpi

Showing: 3701 - 3750 of 116638 results

  1. pRS413-GAL1-luc*(-SKL)

    Plasmid
    #40234
    Depositor
    Michael Benton
    Insert
    luc*(-SKL)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    GAL1
    Available since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    40234-plasmid-map-sequence-id-51354

    Enlarge

  2. pcDNA-SUN2 EGFP

    Plasmid
    #187678
    Purpose
    In vivo visualization of SUN2
    Depositor
    Mary Beckerle
    Insert
    SUN2 (Sun2 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    CMV
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    187678-plasmid-map-sequence-id-371403

    Enlarge

  3. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

    Enlarge

  4. HA-Rab5A

    Plasmid
    #131416
    Purpose
    Expresses HA-tagged human Rab5A in mammalian cells
    Depositor
    Ken-Ichi Takemaru, FENG-QIAN Li
    Insert
    human Rab5A
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131416-plasmid-map-sequence-id-258192

    Enlarge

  5. pOCC102-MBP-eIF4G

    Plasmid
    #219971
    Purpose
    eIF4G protein purification
    Depositor
    Simon Alberti
    Insert
    eIF4G (TIF4631 Budding Yeast)
    Use
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219971-plasmid-map-sequence-id-436844

    Enlarge

  6. QPRTA-c002

    Plasmid
    #205115
    Purpose
    QPRT protein expression
    Depositor
    Paul Brennan
    Insert
    QPRT (QPRT Human)
    Use
    Tags
    TEV-6His
    Expression
    Insect
    Mutation
    WT
    Promoter
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205115-plasmid-map-sequence-id-410409

    Enlarge

  7. pTol2-Synapse reporters

    Plasmid
    #196899
    Purpose
    zebrafish expression
    Depositor
    Reinhard Köster
    Insert
    Synapse reporters
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196899-plasmid-map-sequence-id-386836

    Enlarge

  8. p4286

    Plasmid
    #198152
    Purpose
    bacterial expression of sseJ::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseJ sseJ::L16::HaloTag::HA (sseJ Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198152-plasmid-map-sequence-id-388084

    Enlarge

  9. pJ3 SHP1 C453S

    Plasmid
    #8573
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    C453S (catalytically inactive)
    Promoter
    Available since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  10. pCAG-B19M

    Plasmid
    #59923
    Purpose
    Expression vector for Rabies virus SAD B19 matrix protein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus matrix protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59923-plasmid-map-sequence-id-94982

    Enlarge

  11. pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

    Plasmid
    #205283
    Purpose
    GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)
    Depositor
    Matthias Prigge
    Insert
    GCaMP8f
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205283-plasmid-map-sequence-id-412570

    Enlarge

  12. pCoHygro L16_shRNA of spliced Copia

    Plasmid
    #71389
    Purpose
    knock down expression of spliced Copia in Drosophila melanogaster
    Depositor
    Timothy Nilsen
    Insert
    Copia
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    U6
    Available since
    Nov. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    71389-plasmid-map-sequence-id-129772

    Enlarge

  13. dCas9 gateway compatible

    Plasmid
    #128322
    Purpose
    dCas9 C-terminal gateway cloning vector
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128322-plasmid-map-sequence-id-255033

    Enlarge

  15. mClover-pBAD

    Plasmid
    #54805
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and mClover
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. 4MT-D3-CPV

    Plasmid
    #58184
    Purpose
    Localization: Mitochondria Ca Biosensor, Excitation: 433 / 515 , Emission: 475 / 528
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    ECFP and Venus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. pCAGGS-pac-T2A-dre

    Plasmid
    #124833
    Purpose
    Puromycin resistance gene and dre recombinase
    Depositor
    Mario Capecchi
    Insert
    pac-T2A-dre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    124833-plasmid-map-sequence-id-247139

    Enlarge

  18. pfNL43-dGPE-EGFP

    Plasmid
    #36866
    Depositor
    Mario Chin
    Insert
    NL43-dGPE-EGFP
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    parts of Gag and Pol were deleted and EGFP insert…
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36866-plasmid-map-sequence-id-46081

    Enlarge

  19. Clover2-pBAD

    Plasmid
    #54576
    Purpose
    Localization: Protein Expression Vector , Excitation: 505, Emission: 515
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and Clover2
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pLenti6-DJ1-V5-L166P

    Plasmid
    #29414
    Depositor
    Mark Cookson
    Insert
    DJ1 (PARK7 Human)
    Use
    Lentiviral
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    Leu166Pro
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  21. T7RNAP Cyslight A188C

    Plasmid
    #205083
    Purpose
    T7RNAP protein expression
    Depositor
    Smita Patel
    Insert
    T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C125S, C347S, C510S, C515S, C530S, C723S N88C
    Promoter
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205083-plasmid-map-sequence-id-413708

    Enlarge

  22. pIRES Hygro3 LifeAct-mChe

    Plasmid
    #64343
    Purpose
    Bicistronic expression of LifeAct-mCherry fusion and Hygro resistance gene
    Depositor
    Matthieu Piel
    Insert
    LifeAct
    Use
    Tags
    IRES-Hygro and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. 1437 pSG5L Flag HA PTEN

    Plasmid
    #10786
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Use
    Tags
    Flag and HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 5, 2006
    Availability
    Academic Institutions and Nonprofits only

  24. pSOX2.1.0-gDNA

    Plasmid
    #176382
    Purpose
    Expresses spCas9 and gRNA sequence targeting SOX2
    Depositor
    Kevin White, Barbara Stranger
    Insert
    SOX2 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    176382-plasmid-map-sequence-id-347388

    Enlarge

  25. pPs-neo HA-RalA

    Plasmid
    #20181
    Depositor
    William Hahn
    Insert
    RalA (RALA Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G23V (constitutively active), E147D
    Promoter
    Available since
    March 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    20181-plasmid-map-sequence-id-207517

    Enlarge

  26. TOPOII p48 (DM#261)

    Plasmid
    #15026
    Depositor
    Douglas Melton
    Insert
    p48 (Ptf1a Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  27. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196715-plasmid-map-sequence-id-389692

    Enlarge

  28. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, FreeGenes Project, Jennifer Molloy
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

    Enlarge

  29. pSB-Bleo STAT3

    Plasmid
    #139184
    Purpose
    Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone.
    Depositor
    Neal Devaraj
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    STAT3
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    139184-plasmid-map-sequence-id-309370

    Enlarge

  30. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

    Enlarge

  31. pSP019

    Plasmid
    #168195
    Purpose
    expresses proteolytically inactive EatA passenger domain with H134R mutation within the protease catalytic triad.
    Depositor
    James Fleckenstein
    Insert
    eatA[H134R]
    Use
    Tags
    Expression
    Bacterial
    Mutation
    H134R mutation
    Promoter
    araBAD
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    168195-plasmid-map-sequence-id-339468

    Enlarge

  32. pEF1-His-NEDD8

    Plasmid
    #159322
    Purpose
    Mammalian cell expression of mouse His-NEDD8
    Depositor
    Jianjun Zhao
    Insert
    His-NEDD8 (Nedd8 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159322-plasmid-map-sequence-id-311864

    Enlarge

  33. pAS1 - pRig-3::GCaMP6f::unc-54

    Plasmid
    #194970
    Purpose
    Fluorescent reporter of cytosolic calcium in C.elegans.
    Depositor
    Frederic Hoerndli
    Insert
    GCaMP6f
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    rig-3
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    194970-plasmid-map-sequence-id-388695

    Enlarge

  34. pDEST40-DJ1-C106A

    Plasmid
    #29409
    Depositor
    Mark Cookson
    Insert
    DJ1 (PARK7 Human)
    Use
    Destination vector
    Tags
    6XHis and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. pBSVSG221KOblast

    Plasmid
    #59733
    Purpose
    For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.
    Depositor
    Gloria Rudenko
    Insert
    VSG221 knockout construct
    Use
    For homologous recombination into vsg221 expressi…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    59733-plasmid-map-sequence-id-93744

    Enlarge

  36. ADH1pro_GCN4_SIR4

    Plasmid
    #138620
    Purpose
    Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement
    Depositor
    Rob Mitra
    Insert
    GCN4 (GCN4 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ADH1
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    138620-plasmid-map-sequence-id-334951

    Enlarge

  37. pKM263-EcPPK

    Plasmid
    #38333
    Depositor
    Florian Freimoser
    Insert
    E. coli polyphosphate kinase (ppk Escherichia coli)
    Use
    Tags
    6xHIS, GST, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    38333-plasmid-map-sequence-id-48396

    Enlarge

  38. pY120 (pcDNA3.1-huFbCpf1)

    Plasmid
    #92296
    Purpose
    Expression of humanized FbCpf1
    Depositor
    Feng Zhang
    Insert
    huFbCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    92296-plasmid-map-sequence-id-181502

    Enlarge

  39. FUW-mTie1

    Plasmid
    #119234
    Purpose
    Plasmid encodes full-length murine Tie1 gene cloned in lentiviral vector.
    Depositor
    Kari Alitalo
    Insert
    Tie1 (Tie1 Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    P hUbC
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    119234-plasmid-map-sequence-id-225493

    Enlarge

  40. MT1A

    Plasmid
    #105700
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 1a
    Depositor
    Artur Krężel
    Insert
    Metallothionein 1a (MT1A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105700-plasmid-map-sequence-id-203716

    Enlarge

  41. p4118

    Plasmid
    #198151
    Purpose
    bacterial expression of sseF::HaloTag fusion
    Depositor
    Michael Hensel
    Insert
    PsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)
    Use
    Tags
    HaloTag-HA
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    198151-plasmid-map-sequence-id-388048

    Enlarge

  42. SEMA3E (mouse) pBLUE

    Plasmid
    #29453
    Depositor
    Jonathan Raper
    Insert
    Sema3E (mouse) (Sema3e Mouse)
    Use
    Pblue for ish
    Tags
    Expression
    Mutation
    1400bp fragment for ISH
    Promoter
    Available since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  43. L3691

    Plasmid
    #1587
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    mec7-836, mec7Tsa, T7p, gfpN, IVSR, gfpS65C, IVSS…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1587-plasmid-map-sequence-id-197

    Enlarge

  44. pICSL11085_GG_Wheat_live_Cas9_for

    Plasmid
    #126881
    Purpose
    Encodes a wheat codon optimized Cas9 module in forward direction for golden gate cloning of genome editing binary plasmids.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    126881-plasmid-map-sequence-id-249630

    Enlarge

  45. pJY4092

    Plasmid
    #113334
    Purpose
    Vector for Hermes transposition; Hermes TIR-flanked AvrII cut site; recoded transposase driven by TEF1 promoter
    Depositor
    Suzanne Sandmeyer
    Insert
    codon-optimized Hermes transposase
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    113334-plasmid-map-sequence-id-218285

    Enlarge

  46. pBD001

    Plasmid
    #201330
    Purpose
    pDefence14:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence14:Eluc
    Use
    Tags
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Promoter
    Available since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    201330-plasmid-map-sequence-id-410038

    Enlarge

  47. pGL3 RFPL4b promoter

    Plasmid
    #69250
    Purpose
    Luciferase reporter for Dux4 activation of RFPL4b transcription
    Depositor
    Stephen Tapscott
    Insert
    RFPL4b (RFPL4B Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    RFPL4b
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  48. pJK100

    Plasmid
    #73264
    Purpose
    Produces Bacillus subtilis phosphoribosylformylglycinamidine synthase small subunit (BsPurS)
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylformylglycinamidine synthase subunit PurS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    73264-plasmid-map-sequence-id-134799

    Enlarge

  49. p41Nat MagicFluor

    Plasmid
    #58563
    Purpose
    p41Nat carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58563-plasmid-map-sequence-id-90668

    Enlarge

  50. pGEX4T1-NDFIP2

    Plasmid
    #179330
    Purpose
    E. coli optimized expression of GST-fusion of human NDFIP2
    Depositor
    Arthur Haas
    Insert
    NDFIP2 (NDFIP2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179330-plasmid-map-sequence-id-358705

    Enlarge

Showing: 3701 - 3750 of 116638 results