Addgene

We narrowed to 2,080 results for: 3-Oct

Showing: 1481 - 1500 of 2080 results

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SPOP-V5 (WT)

Plasmid

#169720

Purpose

Mammalian Expression of wild-type mouse SPOP

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-JEDI-1P-Kv-WPRE

Plasmid

#202608

Purpose

Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-Kv

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCVL.Active/Repressed TLR (Sce)

Plasmid

#45575

Purpose

Codes for the Active/Repressed TLR with the target site for I-Sce I nuclease

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Inserts

eGFP with I-Sce I TS
+3 mCherry

Use

Lentiviral

Tags

T2A dislinker and iRFP

Expression

Bacterial and Mammalian

Mutation

embedded I-Sce I TS from 163-185

Promoter

SFFV

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (F133V)

Plasmid

#169725

Purpose

Mammalian Expression of mouse SPOP F133V

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

F133V

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

Plasmid

#171089

Purpose

CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

Depositor

Sebastian Lourido

Article

Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

Inserts

Decoy sgRNA
dCas9
Chloramphenicol acetyltransferase

Use

CRISPR; Toxoplasma gondii expression

Tags

3X FLAG and Nuclear Localization Signal

Expression

Mutation

D10A, H840A

Promoter

TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FRT-JEDI-1P-Kv-WPRE

Plasmid

#202617

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P flanked by FRT in AAV production vector expressed under the mammalian promoter (EF1a), FLP recombinase-dependent

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

FRT-JEDI-1P-Kv

Use

AAV; Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (W131G)

Plasmid

#169724

Purpose

Mammalian Expression of mouse SPOP W131G

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

W131G

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

Plasmid

#69905

Purpose

Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Human, Fly)

Use

Tags

Expression

Mammalian

Mutation

Expresses aa1-194 of HIPK3

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

Plasmid

#202606

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-P2A-mCherry-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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mutpRL-TK 3xUTR

Plasmid

#40758

Purpose

To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

Depositor

Phillip Zamore

Article

Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

Insert

3 copies of the CXCR4 small RNA target site

Use

Luciferase

Tags

luciferase

Expression

Mutation

mutated from TAG to CTC at nt 387–389 of the Reni…

Promoter

TK

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pAGHa-SERBP1(189-400)

Plasmid

#172315

Purpose

Expression of the C-terminal (189-400) domain of SERBP1 in E. coli.

Depositor

David Libich

Article

Baudin et al Biomol NMR Assign. 2021 Oct;15(2):461-46

Insert

Plasminogen Activator Inhibitor 1 RNA-binding protein, residues 189-400 (SERBP1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted 1-188, 401-408

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFL His-SDS22

Plasmid

#113512

Purpose

Expression of His-SDS22 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

SDS22 (PPP1R7 Human)

Use

Tags

6*His

Expression

Insect

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BACK))

Plasmid

#169722

Purpose

Mammalian Expression of mouse SPOP Y353E

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Use

Tags

V5

Expression

Mammalian

Mutation

Y353E

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00517

Plasmid

#39618

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5893 (D Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
IL1RN gRNA3

Plasmid

#113132

Purpose

Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-279

Plasmid

#22707

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

micro RNA 279 (mir-279 primary transcript, Fly)

Use

Drosophila expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00102

Plasmid

#34257

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10388 (Ubx Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00624

Plasmid

#39696

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7450 (CrebA Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyso-Digda

Plasmid

#236102

Purpose

Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

Lyso-Digda (LAMP1 Synthetic, Human)

Use

Tags

Full-length LAMP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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