We narrowed to 6,134 results for: nls
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Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-CibN
Plasmid#60552PurposeExpresses dCas9-CibN in mammalian cellsDepositorInsertdCas9-CibN
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTX179
Plasmid#89261PurposeExpress STU (Single Transcriptional Unit) Cas9 nickase with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ155
Plasmid#69306PurposeGateway entry vector with AteCas9D10ADepositorInsertAteCas9D10A (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NmCas9-FLAG
Plasmid#47872PurposeFLAG tagged NmCas9 with no NLS.DepositorInsertNmCas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAIO-LbCpf1
Plasmid#137847PurposeLbCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.DepositorInsertLbCpf1-NLS-FLAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1748
Plasmid#127982PurposeAMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTX172
Plasmid#89259PurposeExpress STU (Single Transcriptional Unit) Cas9 with Maize Ubiquitin1 promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT15001-AncBE4max
Plasmid#163016Purposeplasmid expressing AncBE4maxDepositorInsertAncBE4max
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTX176
Plasmid#89260PurposeExpress estrodial-inducible STU (Single Transcriptional Unit) Cas9 in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1148-pCSDest-AcrIIA4-mCherry
Plasmid#126204PurposeMammalian expression of AcrIIA4 fused to mCherryDepositorInsertcodon-optimized AcrIIA4
UseTagsFLAG/NLS and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP) mnuctdTomato
Plasmid#172342PurposemnuctdTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTagsSV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQP-AR10
Plasmid#138980PurposeEndogenous RAS regulation using light-controlled intrabodyDepositorInsertBglII-BphP1-NES-T2A- iB(RAS)-NES-mCherry-QPAS1- NLS-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAIO-AsCpf1
Plasmid#137846PurposeAsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.DepositorInsertAsCpf1-NLS-FLAG
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTX168
Plasmid#89257PurposeExpress STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plantsDepositorTypeEmpty backboneUseCRISPRTagsSV40 NLSExpressionPlantMutationPromoterAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ159
Plasmid#69308PurposeGateway entry vector with hSpCas9D10ADepositorInserthSpCas9D10A (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationD10A mutation; NLS fusionPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT9749-dABE
Plasmid#162998Purposeplasmid expressing ABE with dCas9DepositorInsertdABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRG711_lexO-AscI_LexA-TF_HIS3MX
Plasmid#154820PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAT15002-ABEmax
Plasmid#163006Purposeplasmid expressing ABEmaxDepositorInsertABEmax
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15064-eCBE
Plasmid#163009Purposeplasmid expressing eCBEDepositorInserteCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9676-ABE
Plasmid#162997Purposeplasmid expressing ABEDepositorInsertABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PABE-2
Plasmid#115623PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBALU9
Plasmid#69324PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd GFP intron;use C-term after STOPDepositorInsertgSL2-NLS-GFPint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ158
Plasmid#69307PurposeGateway entry vector with hSpCas9 (Human codon optimized)DepositorInserthSpCas9 (Human codon optimized)
UseCRISPRTags3XFLAGExpressionMutationNLS fusionPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDSARN
Plasmid#62295Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBALU12
Plasmid#69328PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2nd mChO intron;use C-term after STOPDepositorInsertgSL2-NLS-mChOint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
nucHCB2
Plasmid#98581PurposeNuclear Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49–148 of huntingtin, fused to eYFP and nuclear localization signal.DepositorInsertHCB2
UseTagsNLS and YFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PABE-6
Plasmid#115627PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-2*NLS
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSAYN
Plasmid#62294Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSLZ
Plasmid#51495PurposepLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmidDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant t-dna plasmidTagsSV40 NLSExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBALU18
Plasmid#69334PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: bicistronic; FRT-galK-FRT in 2ndmChO intron; use C-term after STOPDepositorInsertrSL2-NLS-mChOint-FgF
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIVT-evoBxb1
Plasmid#232747PurposeIVT template for making modRNA encoding evoBxb1DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSExpressionMutationV74APromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1-I87L
Plasmid#232749PurposeIVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSExpressionMutationI87LPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCL390
Plasmid#219500PurposeIntegrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpressionDepositorInsertIntegrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
UseTagsSV40-NLSExpressionYeastMutationPromoterGAL1-GAL10Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL25_Cas9-His Δcys E1207C
Plasmid#206286PurposeBacterial expression of Cas9-His Δcys E1027C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1207CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL23_Cas9-His Δcys E945C
Plasmid#206284PurposeBacterial expression of Cas9-His Δcys E945C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E945CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL24_Cas9-His Δcys E1026C
Plasmid#206285PurposeBacterial expression of Cas9-His Δcys E1026C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, C574S, E1026CPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDL22_Cas9-His Δcys E532C
Plasmid#206283PurposeBacterial expression of Cas9-His Δcys E532C variant under T7 promoterDepositorInsertSpCas9
UseCRISPRTags6x-His and SV40 NLSExpressionBacterialMutationC80S, E532C, C574SPromoterT7Available sinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a no GFP)
Plasmid#172339PurposemnucTomatoDepositorInsertsmonomeric nuclear Tomato
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(EF1a)TetR
Plasmid#172352PurposeTet Repressor reporterDepositorInsertsTetR
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAL1-VP1 (LEU2)
Plasmid#182480PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertdeltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT15065-HF-CBE
Plasmid#163010Purposeplasmid expressing HF-CBEDepositorInsertHF-CBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15066-Hypa-CBE
Plasmid#163011Purposeplasmid expressing Hypa-CBEDepositorInsertHypa-CBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15067-HypaR661A-CBE
Plasmid#163012Purposeplasmid expressing HypaR661A-CBEDepositorInsertHypa-CBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationR661APromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15069-HeF-CBE
Plasmid#163014Purposeplasmid expressing HeF-CBEDepositorInsertHeF-CBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT15000-BE4max
Plasmid#163015Purposeplasmid expressing BE4maxDepositorInserteCBE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9995-evoABE
Plasmid#163003Purposeplasmid expressing evoABEDepositorInsertevoABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT9784-xABE
Plasmid#163005Purposeplasmid expressing xABEDepositorInsertxABE
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailable sinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only