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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,672 results for: pCas

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Showing: 1501 - 1520 of 2672 results

  2. pX335-EN477

    Plasmid
    #86232
    Purpose
    For transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.
    Depositor
    Benoit Bruneau
    Publication
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    spCas9-nickase and sgRNA against mouse CTCF STOP Codon
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86232-plasmid-map-sequence-id-177242

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  3. miniABEmax (pRZ900)

    Plasmid
    #131311
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131311-plasmid-map-sequence-id-254652

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  8. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Publication
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

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  9. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid
    #154430
    Purpose
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Depositor
    David Liu
    Publication
    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:
    Insert
    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    Use
    AAV and CRISPR
    Expression
    Mammalian
    Mutation
    VRQR point mutations in SpCas9
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154430-plasmid-map-sequence-id-304586

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  10. pCMV-Target-AIDmax

    Plasmid
    #139107
    Purpose
    Mammalian Target-AIDmax expression vector (pSI-626)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139107-plasmid-map-sequence-id-296574

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  11. pCY5k

    Plasmid
    #160294
    Purpose
    Yeast CRISPR plasmid targeting the kanMX cassette
    Depositor
    Amir Aharoni
    Publication
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    URA3 selection cassette
    sgRNA expression cassette (without guide)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160294-plasmid-map-sequence-id-464601

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  15. pMIA3-Keppel

    Plasmid
    #214446
    Purpose
    Expresses eSpCas9 and gRNA targeting Keppel-19 GSH
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-P2A-mRuby2-P2A-tp53dn
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214446-plasmid-map-sequence-id-431687

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  20. ABE7.10-F148A

    Plasmid
    #132946
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Publication
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ABE7.10(F148A)
    Use
    CRISPR; Base editor
    Expression
    Mammalian
    Mutation
    ABE7.10(F148A)
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    132946-plasmid-map-sequence-id-314827

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Showing: 1501 - 1520 of 2672 results