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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,315 results for: LEA

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Showing: 1541 - 1560 of 12315 results

  6. U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1

    Plasmid
    #92120
    Purpose
    Expression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-P65-HSF1 (HSF1 Synthetic, Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    92120-plasmid-map-sequence-id-197614

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  9. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Publication
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  10. pcDNA3-Cyto-CaNAR2

    Plasmid
    #64729
    Purpose
    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
    Depositor
    Jin Zhang
    Publication
    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CaNAR2-NES
    Tags
    Cerulean3, Nuclear export signal (NES), and YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64729-plasmid-map-sequence-id-109343

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  11. SpySwitch

    Plasmid
    #184225
    Purpose
    Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpySwitch
    Tags
    His6
    Expression
    Bacterial
    Mutation
    E77A mutation prevents isopeptide bond formation,…
    Promoter
    T7
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    184225-plasmid-map-sequence-id-361604

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  12. Hamdoun lab FP fusion plasmids

    Plasmid kit
    #1000000033
    Purpose
    Generate N- and C-terminal fusions to mCherry, Cerulean, mCitrine, or EGFP for screening fluorescent protein fusion expression in organisms or cells including Zebrafish, Xenopus, and C. elegans.
    Depositor
    Amro Hamdoun
    Application
    Gene Expression and Labeling
    Vector type
    Worm Expression, Xenopus Expression, Zebrafish Expression
    Available since
    July 24, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pAcGP67A - murine TfR

    Plasmid
    #12392
    Depositor
    Pamela Bjorkman
    Insert
    transferrin receptor (Tfrc Mouse)
    Tags
    6X-His tag, Factor Xa cleavage site, and leader p…
    Expression
    Insect
    Mutation
    See comments section
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    12392-plasmid-map-sequence-id-274275

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  14. pcDNA3-cytoEKAR4

    Plasmid
    #174438
    Purpose
    Cytosol-targeted EKAR4.
    Depositor
    Jin Zhang
    Publication
    Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cytoEKAR4
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174438-plasmid-map-sequence-id-367724

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  16. EF13E

    Plasmid
    #42942
    Depositor
    Eline Luning Prak
    Publication
    Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EN- Human L1 element (L1RE1 Human)
    Tags
    EGFP (opposite strand)
    Expression
    Mammalian
    Mutation
    AgeI-BclI fragment swapped with nt 1927-3708 from…
    Promoter
    CMV MIE, L1 5'UTR
    Available since
    April 25, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. pcDNA3-TORCAR-NLS

    Plasmid
    #64931
    Purpose
    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    Tags
    Cerulean, Nuclear localization signal (NLS), and …
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    64931-plasmid-map-sequence-id-109697

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Showing: 1541 - 1560 of 12315 results