We narrowed to 13,592 results for: crispr cas9

1. pIgnaviCas9

   Plasmid

   #127595

   Purpose

   Expresses IgnaviCas9 in E. coli

   Depositor

   Stephen Quake

   Article

   Schmidt et al Proc Natl Acad Sci U S A. 2019 Oct 28. p

   Insert

   IgnaviCas9
   

   Use

   Tags

   6xHis Tag and Maltose binding protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2T_CAG_SpCas9PE2_5plinker_BlastR

   Plasmid

   #173066

   Purpose

   Expresses PE2

   Depositor

   Richard Sherwood

   Article

   Velimirovic et al Nat Commun. 2022 Jun 18;13(1):3512. doi:

   Insert

   5plinker
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRGEN-CMV-CjCas9

   Plasmid

   #89752

   Purpose

   Expression of CjCas9 in mammalian cells

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAH-CTX1-rhadCas9

   Plasmid

   #129391

   Purpose

   Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

   Depositor

   Silvia Cardona

   Article

   Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

   Insert

   Burkholderia cenocepacia codon-optimized dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Entire dCas9 codon optimized for the GC-rich B. c…

   Promoter

   PrhaBAD

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT15547_PEmax-SuperFi-Cas9

   Plasmid

   #184377

   Purpose

   Mammalian expression of SuperFi-Cas9 PEmax prime editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   PEmax-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-myc NLS

   Expression

   Mammalian

   Mutation

   H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas9-beta-mmr

   Plasmid

   #169241

   Purpose

   Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   mutL-E36K
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed glutamic acid 36 to lysine

   Promoter

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAT15546_nABE8e-SuperFi-Cas9

    Plasmid

    #184376

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAT15542_nCBE-SuperFi-Cas9

    Plasmid

    #184372

    Purpose

    Mammalian expression of SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAT15544_nABE-SuperFi-Cas9

    Plasmid

    #184374

    Purpose

    Mammalian expression of SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    nABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-RPT6-FLAG

    Plasmid

    #205416

    Purpose

    Expresses dCas9-RPT6 with FLAG label

    Depositor

    Timothy Jarome

    Article

    Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

    Insert

    dCas9-RPT6 (Psmc5 Rat)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

    Plasmid

    #197421

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2

    Plasmid

    #197422

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

    Plasmid

    #197423

    Purpose

    Knock out of EB3 in human cells by CRISPR/Cas9

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

    Plasmid

    #199253

    Purpose

    Expression construct encoding the high specificity SpCas9-KA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A

    Promoter

    CAG promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge