We narrowed to 11,528 results for: ESP

1. pX459V2.0-eSpCas9(1.1)

   Plasmid

   #108292

   Purpose

   pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

   Depositor

   Yuichiro Miyaoka

   Article

   Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   May 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJMC-L1-Donor-Esp3I

   Plasmid

   #197842

   Purpose

   Donor vector to restore L1 destination via Esp3I

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   None
   

   Use

   Unspecified

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pdg459-eSp(1.1) V3

   Plasmid

   #226965

   Purpose

   CBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pirespuro2-CTLA4-mEos2

   Plasmid

   #98285

   Purpose

   mammalian expression of CTLA4-mEos2

   Depositor

   Mike Heilemann

   Article

   Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

   Insert

   CTLA4 (CTLA4 Human)
   

   Tags

   mEos2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. eSpCas9(1.1)-Puro

   Plasmid

   #117686

   Purpose

   The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.

   Depositor

   Luc Leyns

   Article

   Liu et al Biochem Biophys Res Commun. 2019 Feb 14.

   Insert

   eSpCas9(1.1)
   

   Use

   CRISPR

   Tags

   PuroR (R166H)

   Expression

   Mammalian

   Mutation

   K848A, K1003A, R1060A

   Promoter

   U6

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. H2B-mNeonGreen-IRESpuro2

   Plasmid

   #183745

   Purpose

   Expresses H2B-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIRESpuroN3xFLAG-His-CDK5RAP3

   Plasmid

   #204555

   Purpose

   Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

   Insert

   CDK5RAP3 (AMY2A Human)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX458 eSp(1.1) V3

   Plasmid

   #226963

   Purpose

   CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AW01_pU.CAG.eSpCas9(1.1).rBGpA

   Plasmid

   #199255

   Purpose

   Expression construct encoding the high specificity eSpCas9(1.1) nuclease

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

   Insert

   High specificity eSpCas9(1.1) nuclease
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   K848A K1003A R1060A

   Promoter

   CAG promoter

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRESpuro-Flag-Pol b

    Plasmid

    #23257

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan;8(1):67-79. doi

    Insert

    DNA polymerase beta (POLB Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pIRESpuroN3xFLAG-UFSP2 C302A

    Plasmid

    #204554

    Purpose

    Expresses 3xFLAG-tagged UFSP2 C302A in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Expression

    Mammalian

    Mutation

    C302A

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid

    #86612

    Purpose

    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-R2L3-IRESpacDeltaTKpA

    Plasmid

    #139535

    Purpose

    Provides IRES-pacDeltaTK-pA cassette on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    PacTK
    

    Expression

    Mammalian

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-deSpCas9

    Plasmid

    #92114

    Purpose

    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. P823_eSpCas9(1.1)-RecA

    Plasmid

    #87264

    Purpose

    Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

    Depositor

    Yonglun Luo

    Article

    Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

    Insert

    RecA
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pirespuro2-CD86-mEos2

    Plasmid

    #98284

    Purpose

    mammalian expression of CD86-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CD86 (CD86 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-Ki-67(RASA)-mCherry_IRESpuro2

    Plasmid

    #182921

    Purpose

    Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

    Depositor

    Daniel Gerlich

    Article

    Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

    Insert

    MKI67 (MKI67 Human)
    

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    RASA mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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