We narrowed to 3,140 results for: cat.3

1. pRK5 FLAG membrane-bound lipin-1 (delta200)

   Plasmid

   #120278

   Purpose

   Expression of ER/Golgi-tethered lipin-1 in mammalian cells

   Depositor

   Sirio Dupont

   Article

   Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

   Insert

   Lipin-1 (Lpin1 Mouse)
   

   Use

   Tags

   FLAG and in-frame fusion with the delta200 Golgi-…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOliSpec3'

   Plasmid

   #228550

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. U6-AsCas12f-full-length-sgRNA

   Plasmid

   #204638

   Purpose

   Expression of full-length AsCas12f sgRNA in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   full-length AsCas12f sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPP089

   Plasmid

   #158796

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   CasPhi-3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPP384

   Plasmid

   #158799

   Purpose

   pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

   Depositor

   Jennifer Doudna

   Article

   Pausch et al Science. 2020 Jul 17;369(6501):333-337.

   Insert

   dCasPhi-3 (D413A)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSADB19-dTomato Barcode Library

   Pooled Library

   #227671

   Purpose

   RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus.

   Depositor

   Tomasz Nowakowski, Cathryn Cadwell

   Article

   Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

   Expression

   Mammalian

   Species

   Synthetic

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

7. GG-EBNA-OCT4

   Plasmid

   #69537

   Purpose

   Episomal plasmid encoding 5 gRNAS targeting human OCT4 promoter

   Depositor

   Timo Otonkoski

   Article

   Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

   Insert

   5 concatenated gRNA transcriptional cassettes targeting OCT4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV3TAG8li-hSTT3A-3FLAG

   Plasmid

   #159140

   Purpose

   Hu STT3A -flag mammalian expression vector.

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   STT3 oligosaccharyltransferase complex catalytic subunit A (STT3A Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAtCHYb1Δ129N

   Plasmid

   #53367

   Purpose

   Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

   Insert

   chyB1 (BETA-OHASE 1 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Deleted codons for the first 129 amino acids

   Promoter

   Trc

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_TEAD3_1

    Plasmid

    #86337

    Purpose

    Encodes gRNA for 3' target of human TEAD3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TEAD3 (TEAD3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. plentiCRISPR-sgMDH1

    Plasmid

    #72875

    Purpose

    The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    sgMDH1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

12. gRNA[bTub].1026C

    Plasmid

    #112691

    Purpose

    express gRNA targeting bTub under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXL_dSSN3_EF1a-tNGFR-2A-Puro

    Plasmid

    #201113

    Purpose

    Dual barcoding cassettes for SSN-seq; Barcode Set 3

    Depositor

    Pawel Mazur

    Article

    Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

    Inserts

    SSN.guide
    SSN.F30
    tNGFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a, Human U6, and Mouse U6

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KBB106

    Plasmid

    #185065

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

    Depositor

    Ken Belanger

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal region, truncated so C-terminal h…

    Promoter

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNAM-B1

    Plasmid

    #154056

    Purpose

    Level 0 Golden Gate vector, contains domesticated NAM-B1

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    TaNAM-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Synonymous mutation at base 3257, in exon 3, to r…

    Promoter

    N/A

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLz75_CB_operon_CsoS2_N3-N4

    Plasmid

    #140857

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1 and N2 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N2), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-109 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLz76_CB_operon_CsoS2_N4

    Plasmid

    #140858

    Purpose

    Same as pLz37 but with CsoS2 truncated such that NTD repeats N1, N2, and N3 are removed

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CbbL, CbbS, CsoS2 (delN1-N3), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-189 of CsoS2

    Promoter

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX300A-G+Nanog

    Plasmid

    #140280

    Purpose

    CRISPR/Cas9 plasmid encoding Cas9 and sgRNA against gBait and Nanog locus

    Depositor

    Yusuke Miyanari

    Article

    Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p40deltaPX (w/out aa1-135)

    Plasmid

    #19019

    Depositor

    Michael Yaffe

    Article

    Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

    Insert

    p40phox (NCF4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa136-148 (PX domain, truncated)

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pKLV2-U6gRNA5(CXCR4-E1(15))-PGKpuro2ABFP-W

    Plasmid

    #200478

    Purpose

    Lentiviral vector expressing gRNA targeting human CXCR4-E1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    CXCR4-E1(15) (CXCR4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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