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Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-imp α
Plasmid#119718PurposeExpresses EGFP-tagged human importinα in mammalian cellsDepositorInsertimportinα (KPNA2 Human)
UseTagsEGFPExpressionMammalianMutationcontains amino acids 251-529PromoterCMVAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NHERF1-FL
Plasmid#28291DepositorInsertNHERF1 (NHERF1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMS178
Plasmid#215678PurposeEmpty repair plasmid for ChrII split hygromycinR landing padDepositorInsert5'HA + MCS + loxP + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMS114
Plasmid#215677PurposeEmpty repair plasmid for ChrI split hygromycinR landing padDepositorInsert5'HA + MCS + loxN + rps-0p::5'ΔHygR
UseCRISPR and Cre/LoxTagsExpressionWormMutation5' ∆HYGR encodes aa1-226PromoterAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-MUH
Plasmid#26213DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRSET-WHaloCaMP1a
Plasmid#205304PurposeExpression of WHaloCaMP1a in E. coliDepositorInsertWHaloCaMP1a
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4-mammalian-recoded_mCherry_pcDNA3.1
Plasmid#114595PurposeEncodes mCherry-tagged BcLOV4 for expression in mammalian cellsDepositorInsertBcLOV4
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213915-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiLAMP5e3_ChR2_mCherry (#213915). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the Lamp5 interneuron-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR
Plasmid#113348PurposeA sgRNA expression plasmid for genome editing in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialMutationPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKV060
Plasmid#133315PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+DepositorInsertLuc+
UseTagsExpressionPlantMutationPromoterAtUbi10Available sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBA620
Plasmid#186240PurposeLbuCas13a Negative Control (RFP targeting)DepositorInsertpTet-eLbuCas13a + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMM113
Plasmid#127210PurposeBeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertWUS2, AtSTM, sgRNA, Luc
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-BMPR1A
Plasmid#23618DepositorInsertBMPR1A (BMPR1A Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
Twitch-2B pRSETB
Plasmid#48203PurposeFluorescent reporter for calcium signalingDepositorInsertTwitch-2B
UseTags6xHIS, cpVenusCD, and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RAM-d2TTA::TRE-ArchT-WPREpA
Plasmid#84472PurposepAAV-RAM-ArchTDepositorInsertArchT
UseAAVTagsEGFPExpressionMutationPromoterTREAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-FLAG-mISG15
Plasmid#12443DepositorInsertISG15
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Raichu-454HRasCT
Plasmid#176029PurposeA FRET biosensor for HRas activityDepositorInsertRaichu-Ras-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only