179,202 results
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Plasmid#172878Purposeexpresses ultraID in mammalian cells with the tet-on or tet-off systemDepositorInsertultraID
UseLuciferase and Retroviral; To be used in a tet-on…TagsmycExpressionMammalianMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterTRE-cmvAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (AAV9)
Viral prep#108912-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 (#108912). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 plasmid DNA. CAG-driven, Cre-dependent ChroME-ST-P2A-H2B-mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3 (Cre-dependent)Available sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-ATF4 mScarlet NLS
Plasmid#115969PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-IDepositorInsertactivating transcription factor 4 (ATF4 Synthetic, Human)
UseLentiviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_AncBE4max
Plasmid#112094PurposeC:G-to-T:A base editingDepositorInsertAncBE4max
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
miniTurbo-His6_pET21a
Plasmid#107178Purposeexpresses His6-tagged miniTurbo in bacteriaDepositorInsertminiTurbo (BirA mutant)
ExpressionBacterialMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Available sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCasSA
Plasmid#98211PurposeCRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK293 (mAID-mCherry2-Hygro)
Plasmid#72831PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorInsertmAID-mCherry2-Hygro
UseCRISPRTagsmAID-mCherry2ExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330S-2
Plasmid#58778PurposeExpresses Cas9 nuclease and gRNADepositorInserthumanized S. pyogenes Cas9 nuclease
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-R-GECO1.2
Plasmid#45494PurposeRed intensiometric genetically encoded Ca2+-indicators for optical imaging 1.2DepositorPublicationInsertR-GECO1.2
ExpressionMammalianMutationR-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…PromoterCMVAvailable sinceJuly 23, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Tol2
Plasmid#31823DepositorInsertTol2 Transposase
ExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-myc3-CUL4B
Plasmid#19922DepositorPublicationAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-CD14
Plasmid#13645DepositorInsertCD14 (CD14 Human)
ExpressionMammalianAvailable sinceJune 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cdk5-HA
Plasmid#1872DepositorAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lck-mScarlet-I
Plasmid#98821PurposeIn vivo visualization of the plasma membrane (can be used for colocalization studies)DepositorInsertLck
TagsmScarlet-IExpressionMammalianPromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-MBP-TEV
Plasmid#69929Purposebacterial expression of MBP-TEV n-terminal fusionsDepositorPublicationTypeEmpty backboneTagsMBP and TEV cleavage siteExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJan. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pULTRA-CNF
Plasmid#48215Purposehighly efficient suppressor plasmidDepositorInsertspolyspecific MJ tyrosyl synthetase
tRNA
ExpressionBacterialMutationY32L, L65V, F108W, Q109M, D158G, I159APromoterproK and tacIAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-LUC2CP/intron/ARE
Plasmid#62858Purposesplicing reporter (with intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseExpressionMammalianMutationinsertion of chimeric intron at nueotide poclsiti…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET-MBP-mSA2
Plasmid#52319PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, MBP, and TEVExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
ExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Peft-3::cas9-SV40_NLS::tbb-2 3'UTR
Plasmid#46168PurposeCodon optimized Cas9_SV40 NLS with intronDepositorInsertcodon optimized Cas9_SV40 NLS with intron
UseCRISPRMutationintron inserted at position 3113-3163, and SV40 N…Promotereef-1A.1 (eft-3)Available sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by