166,779 results

1. pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

   Plasmid

   #231721

   Purpose

   Protein expression in insect cells

   Depositor

   Jesper Svejstrup

   Article

   Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   Flag-GST-3C

   Expression

   Insect

   Mutation

   RPB1:1586-1971

   Promoter

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNX24-RFP-MS2SL

   Plasmid

   #227819

   Purpose

   Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Inserts

   3xHA-RFP-24xMS2SL
   Neo resistance
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SV40

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EF1a-KLF2-T2A-mCHERRY

   Plasmid

   #224295

   Purpose

   Expresses mouse KLF2 and mCherry under EF1a promoter

   Depositor

   Nikhil Joshi

   Article

   Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

   Insert

   KLF2 T2A mCherry (Klf2 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGEN-Dre-nls (JDW 1131)

   Plasmid

   #229809

   Purpose

   A CAGGS-driven dre recombinase followed by a c-terminal 1x nls sequence

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DRE recombinase-nls
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AiP13030 - pAAV-AiE0462m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3030)

   Plasmid

   #229909

   Purpose

   AiE0462m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

   Plasmid

   #213527

   Purpose

   Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   D90A and E92A

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRSF_his6_sumo_OC43_NSP14

   Plasmid

   #213519

   Purpose

   Expresses NSP14 of hCoV OC43

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV OC43 NSP14 (ORF1ab hCoV OC43)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRSF_his6_sumo_NL63_NSP14

   Plasmid

   #213520

   Purpose

   Expresses NSP14 of hCoV NL63

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV NL63 NSP14 (ORF1ab hCoV NL63)
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSF_his6_sumo_229E_NSP14

   Plasmid

   #213521

   Purpose

   Expresses NSP14 of hCoV 229E

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV 229E NSP14
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS416-ADH1p-TID-FKBP-HA3

    Plasmid

    #227998

    Purpose

    Expresses TurboID-FKBP fusion from ADH1 promoter; yeast BioID

    Depositor

    Joseph Reese

    Article

    Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

    Insert

    TurboID-FKBP
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUdOm2.1

    Plasmid

    #210278

    Purpose

    Empty backbone with ampicillin resistance for high expression in mammalian cells

    Depositor

    Corrie daCosta

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMK484 (2mAID-Hygro)

    Plasmid

    #214396

    Purpose

    2mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    2mAID-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMK487 (Hygro-P2A-3mAID)

    Plasmid

    #214399

    Purpose

    3mAID for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-3mAID
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMK450 (BromoTag-mCherry-BSD)

    Plasmid

    #214380

    Purpose

    BromoTag-mCherry for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

    Plasmid

    #169826

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A S1859A; TCCC -> AGTC silent mutat…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRAR074-control-sfGFP

    Plasmid

    #185032

    Purpose

    J23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

    Depositor

    Julius Lucks

    Article

    Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

    Insert

    L31-sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Nucleotides of rpmE 5'UTR scrambled, Only fi…

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CL20_mEGFP_KMT2A_AFDN

    Plasmid

    #205840

    Purpose

    Express mEGFP-tagged fusion protein, KMT2A_AFDN from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    KMT2A_AFDN (KMT2A, AFDN Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. psiCHECK2-miR-503-5p

    Plasmid

    #135725

    Purpose

    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-503-5p)

    Depositor

    Florian Karreth

    Insert

    miR-503-5p reverse complement
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWZL Neo Myr Flag VRK3

    Plasmid

    #20664

    Depositor

    Jean Zhao, William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    VRK3 (VRK3 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

20. Inzolia library in pRDA_550

    Pooled Library

    #209552

    Purpose

    The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

    Depositor

    John Doench

    Article

    Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only