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gRNA_CloningVector_miRFP670

Plasmid

#163748

Purpose

Empty gRNA cloning vector, contains a miRFP670 fluorescent marker for FACS sorting after transfection.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBbE15c-RFP

Plasmid

#126168

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS9c-RFP

Plasmid

#126184

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23113

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbS20a-RFP

Plasmid

#126192

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE14k-RFP

Plasmid

#126153

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE19a-RFP

Plasmid

#126161

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE13c-RFP

Plasmid

#126158

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23102

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbE12a-RFP

Plasmid

#126145

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA12a-RFP

Plasmid

#126085

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0548

Plasmid

#162299

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0545

Plasmid

#162296

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0542

Plasmid

#162293

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLPhygSaCas9

Plasmid

#123262

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

Staphylococcus aureus Cas9 (NEWENTRY )

Use

CRISPR; Leishmania

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSPneoSagRNAH

Plasmid

#123265

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNeoSagRNAH

Plasmid

#123266

Purpose

Expresses gRNA of Staphylococcus aureus Cas9 (SaCas9) in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Type

Empty backbone

Use

CRISPR; Leishmania expression

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA18c-RFP

Plasmid

#126111

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23117

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA20c-RFP

Plasmid

#126110

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA19c-RFP

Plasmid

#126109

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23105

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA12c-RFP

Plasmid

#126097

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23151

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA11c-RFP

Plasmid

#126096

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23150

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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