We narrowed to 18,123 results for: Met;

1. pDONR223_LacZ

   Plasmid

   #25893

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   LacZ
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_BFP

   Plasmid

   #25891

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   BFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pXAT2

   Plasmid

   #80494

   Purpose

   AAVS1 sgRNA expression vector

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 sgRNA-T2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBR_attB(bxb)_lox

   Plasmid

   #183762

   Purpose

   Bxb1-specific donor plasmid for the cloning of payloads <20 kb

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-U6-sgRNA-PGK-puromycin

   Plasmid

   #51133

   Purpose

   expresses sgRNA from the U6 promoter.

   Depositor

   Xingxu Huang

   Article

   Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LCC-ICCG_pET21b

   Plasmid

   #215408

   Purpose

   Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   LCC-ICCG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   F243I/D238C/S283C/Y127G

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6_(GLuc)_INT[3xMS2_SL]

   Plasmid

   #68426

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing three MS2 Stem-loops
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR221_EGFP

   Plasmid

   #25899

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   EGFP
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Str-KDEL_TNF-SBP-EGFP

   Plasmid

   #65278

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Str-KDEL_ST-SBP-EGFP

    Plasmid

    #65264

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETM6-E12-vioABECD

    Plasmid

    #66537

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. peCALWY-4

    Plasmid

    #22236

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-4 Zn(II) sensor (ATOX1 GFP's, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C418S mutation linker consists of 9 GGSGGS repea…

    Promoter

    Available since

    Nov. 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSFDuet-1_VNp6-mNeongreen

    Plasmid

    #182388

    Purpose

    Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp6-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSFDuet-1_VNp15-mNeongreen

    Plasmid

    #182389

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti_CamKIIa_GFP

    Plasmid

    #96941

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Ricardo Dolmetsch, Oleksandr Shcheglovitov

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    Green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FH-TET3-pEF

    Plasmid

    #49446

    Purpose

    This plasmid expresses full-length human TET3.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    Tet methylcytosine dioxygenase 3 (TET3 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-vioABECD

    Plasmid

    #66535

    Purpose

    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with consensus promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Inserts

    VioA
    VioB
    VioE
    VioC
    VioD
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Consensus T7

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAVS1-P-CAG-mCh

    Plasmid

    #80492

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    mCherry
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p15_attB(bxb)_lox

    Plasmid

    #183764

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads (~10 - 50 kb)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSupAR-MbPylRS(DiZPK)

    Plasmid

    #91705

    Purpose

    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

    Depositor

    P. Chen

    Article

    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

    Inserts

    pylS
    pylT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG_mRuby2_smFP FLAG

    Plasmid

    #59760

    Purpose

    encodes multiple copies of an epitope tag FLAG ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Insert

    mRuby2_smFP_FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSFDuet-1_hGH

    Plasmid

    #182410

    Purpose

    Bacterial expression of human growth hormone

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    hGH (GH1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRSFDuet-1_VNp-mNeongreen

    Plasmid

    #182387

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-His
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Str-KDEL_TNF-SBP-mCherry

    Plasmid

    #65279

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21-pelB-LaM-4

    Plasmid

    #172770

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-4
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Actin Binding Domain Tensionless Control

    Plasmid

    #215371

    Purpose

    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).

    Depositor

    Brenton Hoffman

    Article

    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    Actin Binding Domain Tensionless Control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAVS1-Nst-MCS

    Plasmid

    #80487

    Purpose

    donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX303

    Plasmid

    #25897

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pc3.1-CAGGS-ASAP3-mCyRFP3

    Plasmid

    #193323

    Purpose

    A red fluorescent protein with improved monomericity enables ratiometric voltage imaging with ASAP3

    Depositor

    Michael Lin

    Article

    Kim et al Sci Rep. 2022 Mar 7;12(1):3678. doi: 10.

    Insert

    ASAP3-R3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

34. pAAVS1-Nst-CAG-DEST

    Plasmid

    #80489

    Purpose

    Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCDH_Str-KDEL_ManII-SBP-mCherry

    Plasmid

    #65259

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Expression

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Profiline

    Plasmid

    #89925

    Purpose

    Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin

    Depositor

    Guillaume Romet-Lemonne, Antoine Jégou

    Article

    Jegou et al PLoS Biol. 2011 Sep;9(9):e1001161. doi:

    Insert

    profiline-1 (PFN1 Human)
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge