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Showing: 1661 - 1680 of 17468 results

  1. pGFP_6

    Plasmid
    #238194
    Purpose
    Empty lentiviral vector for expressing protein sequences fused to GFP-6mer_oligomerization_domain, under a TRE3GV promoter
    Depositor
    Feng Zhang
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    GFP-6mer and SV40
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238194-plasmid-map-sequence-id-472655

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  2. PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2

    Plasmid
    #129629
    Purpose
    Visualization of ERK activity
    Depositor
    Dorus Gadella
    Insert
    PB-H2A-LSSmOrange2-2A-ERK-KTR-LSS-SGFP2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    129629-plasmid-map-sequence-id-260251

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  3. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

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  4. pLZRS-mVenus-TrkC

    Plasmid
    #182948
    Purpose
    To express the rat TrkC fused in the N-terminus with mVenus
    Depositor
    Pantelis Tsoulfas
    Insert
    mVenus-TrkC (Ntrk3 Synthetic, Rat)
    Use
    Retroviral and Synthetic Biology
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MMLV
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182948-plasmid-map-sequence-id-360203

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  5. LentiCRISPR_Hs_MBD3_ex2

    Plasmid
    #62623
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. LentiCRISPR_Hs_MBD3_ex3

    Plasmid
    #62624
    Purpose
    Encodes gRNA which targets human MBD3
    Depositor
    Paul Wade
    Insert
    MBD3 gRNA (MBD3 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Michele Pagano
    Article
    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Promoter
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    172603-plasmid-map-sequence-id-340216

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  9. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  10. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208044-plasmid-map-sequence-id-410266

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  18. LCV2_EGFP

    Plasmid
    #155098
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and EGFP expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155098-plasmid-map-sequence-id-307983

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  19. LCV2_mCherry

    Plasmid
    #155096
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mCherry expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155096-plasmid-map-sequence-id-307998

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  20. LCV2_mClover3

    Plasmid
    #155095
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and mClover3 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155095-plasmid-map-sequence-id-308012

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Showing: 1661 - 1680 of 17468 results