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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,905 results for: NUC

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Showing: 1681 - 1700 of 12905 results

  4. TFORF2973

    Plasmid
    #144449
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARD (PPARD Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    144449-plasmid-map-sequence-id-407332

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  7. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  13. pETDuet-1_VNp-LZ-VenusN154_VNp-LZ-VenusC155 (BiFC construct)

    Plasmid
    #182395
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-amino-half_mVenus BIFC fragment n and Vesicle Nucleating peptide-Leucine Zipper-carboxyl-half_mVenus BIFC fragment fusions
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-VenusN154
    VNp-LZ-VenusC155
    Tags
    Vesicle Nucleating peptide (VNp) & Leucine Zi…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182395-plasmid-map-sequence-id-364922

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  14. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Article
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available Since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109428-plasmid-map-sequence-id-212894

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  18. pET19-MTdTc302

    Plasmid
    #207458
    Purpose
    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302
    Depositor
    Jay Keasling
    Article
    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.
    Insert
    MTdTc302 (Dntt Mouse)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser
    Available Since
    Sept. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    207458-plasmid-map-sequence-id-407772

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  19. pTXB1-EXO1b D173A

    Plasmid
    #68269
    Purpose
    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant
    Depositor
    Stefano Ferrari
    Article
    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10
    Insert
    EXO1 (EXO1 Human)
    Tags
    Mxe intein/chitin binding domain
    Expression
    Bacterial
    Mutation
    catalytically-dead D173A. Please see depositor co…
    Promoter
    T7
    Available Since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. TFORF1965

    Plasmid
    #143560
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PPARD (PPARD Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143560-plasmid-map-sequence-id-400379

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Showing: 1681 - 1700 of 12905 results