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Showing: 2551 - 2580 of 160228 results

  2. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV1)

    Viral Prep
    #26975-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    CaMKII
    Tags
    mCherry
    Available since
    Feb. 15, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. TRUPATH

    Plasmid Kit
    #1000000163
    Purpose
    A collection of 20 plasmids encoding alpha, beta, or gamma subunits of the heterotrimeric G protein complex for detection of heterotrimer dissociation. GPCR
    Depositor
    Bryan Roth
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Promoter
    Available since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  8. pAAV-Ef1a-DIO eNpHR 3.0-EYFP (AAV9)

    Viral Prep
    #26966-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-Ef1a-DIO eNpHR 3.0-EYFP (#26966). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO eNpHR 3.0-EYFP plasmid DNA. EF1a-driven, cre-dependent eNpHR 3.0-EYFP for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.
    Promoter
    EF1a
    Tags
    EYFP (Cre-dependent)
    Available since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  9. pAAV-Ef1a-Coff/Fon-sRGECO (AAV8)

    Viral Prep
    #137128-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Coff/Fon-sRGECO (#137128). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Coff/Fon-sRGECO plasmid DNA. EF1a-driven, Flp-dependent expression of cytosolic sRGECO calcium sensor (inhibited in presence of Cre). These AAV preparations are suitable purity for injection into animals.
    Depositor
    INTRSECT 2.0 Project, Karl Deisseroth
    Article
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    None
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pAAV-hSyn-DIO-mCherry (AAV Retrograde)

    Viral Prep
    #50459-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  11. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV9)

    Viral Prep
    #105530-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available since
    July 13, 2018
    Availability
    Academic Institutions and Nonprofits only

  13. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  14. M13cp-dg3 hp

    Plasmid
    #218095
    Purpose
    Helper plasmid that allows phagemid production upon complementation with a phagemid expressing pIII
    Depositor
    Kevin Esvelt
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    218095-plasmid-map-sequence-id-432463

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  20. pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV5)

    Viral Prep
    #105677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    Syn
    Tags
    FusionRed (Cre-dependent)
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  22. kiCAP-AAV-MyoAAV4A

    Plasmid
    #224448
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYNSL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224448-plasmid-map-sequence-id-446642

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  24. pEG BacMam

    Plasmid
    #160451
    Purpose
    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein
    Depositor
    Eric Gouaux
    Article
    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160451-plasmid-map-sequence-id-319250

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  25. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129591-plasmid-map-sequence-id-254269

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  29. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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Showing: 2551 - 2580 of 160228 results