276,728 results

1. pDONR221_SLC5A9

   Plasmid

   #132230

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A9 (SLC5A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pLV-CBA-dt/PGK-CFTR-⌀

   Plasmid

   #163923

   Purpose

   Expresses human CFTR.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   CFTR (CFTR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-CBA-dt/PGK-CFTR-⌀

   Plasmid

   #163923

   Purpose

   Expresses human CFTR.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   CFTR (CFTR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJM105: pTac RFP on pCM66T

   Plasmid

   #74746

   Purpose

   pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJM105: pTac RFP on pCM66T

   Plasmid

   #74746

   Purpose

   pCM66T with promoter pTac driving RFP expression (maxRFP = BBa_E1010)

   Depositor

   Mary Lidstrom

   Insert

   RFP (BioBrick part number BBa_E1010)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTac

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. JB82

   Plasmid

   #128911

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab10TN-BirA*-HA-MAO (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. JB82

   Plasmid

   #128911

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab10TN-BirA*-HA-MAO (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JB81

   Plasmid

   #128910

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab10QL-BirA*-HA-MAO (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. JB81

   Plasmid

   #128910

   Purpose

   mammalian expression, proximity labelling, mitochondrial relocalisation

   Depositor

   Sean Munro

   Article

   Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

   Insert

   Rab10QL-BirA*-HA-MAO (RAB10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTK_TFAP2a_enh_249_Cerulean

    Plasmid

    #130615

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    TFAP2a_enh_249
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTK_TFAP2a_enh_249_Cerulean

    Plasmid

    #130615

    Purpose

    enhancer reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    TFAP2a_enh_249
    

    Use

    Enhancer reporter

    Tags

    Expression

    Mutation

    Promoter

    thymidine kinase minimal promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR223-ACVR1B

    Plasmid

    #23567

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR1B (ACVR1B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDONR223-ACVR1B

    Plasmid

    #23567

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ACVR1B (ACVR1B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

14. D1433 his3::LYS2 Disruptor Converter

    Plasmid

    #51676

    Purpose

    Yeast marker swap plasmid for converting HIS3 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. D1433 his3::LYS2 Disruptor Converter

    Plasmid

    #51676

    Purpose

    Yeast marker swap plasmid for converting HIS3 to LYS2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    LYS2 (LYS2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLsCas13a-gRNA-NT

    Plasmid

    #164857

    Purpose

    Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    LsCas13a-gRNA-NT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRG217

    Plasmid

    #64531

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRG217

    Plasmid

    #64531

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS313-PRP8

    Plasmid

    #89966

    Purpose

    complements prp8 genomic deletion

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS313-PRP8

    Plasmid

    #89966

    Purpose

    complements prp8 genomic deletion

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    none

    Promoter

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-MMLVgag-D3A-3L-dCas9-ZIM3

    Plasmid

    #240531

    Purpose

    Expresses MMLVgag–DNMT3A-3L-dCas9-ZIM3 for producing RENDER-DNMT3A-3L-dCas9-ZIM3

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-D3A-3L-dCas9-ZIM3
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-MMLVgag-dCas9-ZIM3

    Plasmid

    #240527

    Purpose

    Expresses MMLVgag–dCas9-ZIM3 for producing RENDER-dCas9-ZIM3

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    MMLVgag-dCas9-ZIM3
    

    Use

    Tags

    FLAG, HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OPEN-HLA-B*07:02-BirA

    Plasmid

    #239752

    Purpose

    E. coli expression of BirA tagged HLA-B*07:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-B*07:02 (HLA-B Human)
    

    Use

    Tags

    BirA substrate peptide

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    T7

    Available since

    Aug. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TRBV6-4

    Plasmid

    #237916

    Purpose

    Encodes TRBV6-4 allele and TRAC to generate TCRs via cloning

    Depositor

    Michael Birnbaum

    Insert

    TRBV6-4-TRAC (TRBV6-4 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-mito-GreenPy1High

    Plasmid

    #239070

    Purpose

    Mammalian expression of mitochondria-targeted high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-mito-GreenPy1Lowest

    Plasmid

    #239072

    Purpose

    Mammalian expression of mitochondria-targeted lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Lowest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD-GreenPy1Highest

    Plasmid

    #239049

    Purpose

    Bacterial expression of highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA-mito-GreenPy1Low

    Plasmid

    #239071

    Purpose

    Mammalian expression of mitochondria-targeted low affinity green fluorescent pyruvate biosensor GreenPy1Low

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Low
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-GreenPy1High

    Plasmid

    #239060

    Purpose

    Mammalian expression of high affinity green fluorescent pyruvate biosensor GreenPy1High

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1High
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge