We narrowed to 11,196 results for: ena

1. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

   Plasmid

   #127357

   Purpose

   gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Mutation

   R118G

   Promoter

   no promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLiP-G1

   Plasmid

   #188963

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: atcggtcgcattgttttccactagg
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF-XMAS-2xStop

   Plasmid

   #164412

   Purpose

   Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

   Depositor

   Xiao Wang

   Article

   Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

   Insert

   mCherry
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEY79

   Plasmid

   #191074

   Purpose

   flp-12 fluorescent neural reporter driving nuclear T-Sapphire expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   flp-12 (flp-12 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEY62

   Plasmid

   #191020

   Purpose

   acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-5 (acr-5 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   None

   Promoter

   Available since

   Nov. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMOD_A0801

   Plasmid

   #91022

   Purpose

   Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_dead (D10A + H840A)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, H840A

   Promoter

   35S

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO.1-sh-mSox10-2

   Plasmid

   #37007

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSox10-2 (Sox10 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 31, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pGEX-parkin 219-465

   Plasmid

   #45972

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 219-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti CRISPR sgSHMT1_1

   Plasmid

   #106311

   Purpose

   Express Cas9 and sgRNA targeting SHMT1

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   sgRNA targeting SHMT1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTC365

    Plasmid

    #91214

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, tRNA processing)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mRFP-dmBACCS2

    Plasmid

    #67630

    Purpose

    Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    mRFP-dmBACCS2
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Drosophila actin gene 5C gene promoter

    Available since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCR-Blunt II-TOPO-PTPRD

    Plasmid

    #29705

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pMXs_FLAG-ODC1

    Plasmid

    #133256

    Purpose

    Express FLAG-tagged ODC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC1 (ODC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DCT.VV.Orf137

    Plasmid

    #12825

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf137
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pMOD_A1610

    Plasmid

    #91038

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_D10A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_D10A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJMP1102

    Plasmid

    #119246

    Purpose

    knockdown phzA1 in P. aeruginosa

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA phzA1 (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJMP1103

    Plasmid

    #119247

    Purpose

    knockdown phzM in P. aeruginosa

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA phzM (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJ414-PCY1

    Plasmid

    #98150

    Purpose

    Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBZ191-pU6-sgBFP-CBh-sv40NLS-Cas9-NLS-T2A-mCherry

    Plasmid

    #170183

    Purpose

    Expression vector for a sgRNA against BFP and spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    sgBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1055

    Plasmid

    #119242

    Purpose

    construction intermediate

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCRRNA-G20-R20

    Plasmid

    #101822

    Purpose

    Repress mCherry and GFP independently, with different strengths

    Depositor

    Sven van Teeffelen, David Bikard

    Article

    Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

    Insert

    crRNAs targeting GFP and mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Spy 1049

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTaCas9_6

    Plasmid

    #91169

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. E0030 (YFP)_CD

    Plasmid

    #66031

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Yellow. [C:E0030:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p-mCherry2-sgC9cen

    Plasmid

    #198326

    Purpose

    Expresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    Chr9-CEN guide RNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Empty Anchor Control

    Plasmid

    #127665

    Purpose

    Encodes for puromycin resistance expressed from the EF1a promoter

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Insert

    Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPKm-118

    Plasmid

    #90491

    Purpose

    Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    pFR Luciferase Fluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCS5

    Plasmid

    #55750

    Purpose

    Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    KanR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Residues 59-264 of KanR

    Promoter

    T7-lac

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDIRECT_10A

    Plasmid

    #91207

    Purpose

    Direct Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLY239

    Plasmid

    #184152

    Purpose

    Reprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-R3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLY273

    Plasmid

    #184155

    Purpose

    Reprogrammed short tracrRNA for hijacking mRNA of zraP

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

    Insert

    s-tracrRNA-zraP-22
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJH3666

    Plasmid

    #191447

    Purpose

    Pacr-2s-GCaMP6s::wCherry unc-54 3' UTR C.elegans A/B MN expression of GCaMP6s RFP

    Depositor

    Mei Zhen

    Article

    Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

    Insert

    GCaMP6s
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pacr-2s

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

    Plasmid

    #125227

    Purpose

    Expresses SPARK DRD1 transmembrane component in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CAV1-mEGFP/FRT/TO

    Plasmid

    #27762

    Depositor

    Ari Helenius

    Article

    Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

    Insert

    caveolin-1 (CAV1 Canis lupus familiaris)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEY68

    Plasmid

    #191065

    Purpose

    ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    ceh-14 (ceh-14 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CTA2-mNG211

    Plasmid

    #141167

    Purpose

    Expresses GST-SUMO tagged CTA2 Subunit with 11th Strand

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    SUMO-CTA2-mNG211
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMOD_A1810

    Plasmid

    #91040

    Purpose

    Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_dead (D10A + H840A), Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-TaCas9_dead (D10A + H840A)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, H840A

    Promoter

    ZmUbi

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

40. pJMP1239

    Plasmid

    #119263

    Purpose

    knockdown folA in P. aeruginosa

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge