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pAAV-CAG-NLS-GFP

Plasmid

#104061

Purpose

AAV expression of nuclear localized GFP with SV40 NLS

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB

Insert

GFP

Use

AAV

Promoter

CAG

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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BcLOV4 fusion tools screening plasmid #2

Plasmid

#174512

Purpose

Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

Insert

plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP

Tags

3xFLAG and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-EGFP

Plasmid

#75159

Purpose

Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFLP-hyg

Plasmid

#87831

Purpose

Expresses FLP recombinase with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

heat-shock inducible

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

Plasmid

#98930

Purpose

AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-M-2xStrep-IRES-Puro

Plasmid

#141386

Purpose

Lentiviral expression of SARS-CoV-2 membrane (M) protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-M (M SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

Plasmid

#138151

Purpose

For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

hU6_Rfxcas13d-DR_BsmBI

Use

Lentiviral

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP7f-WPRE

Plasmid

#104488

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV8, and AAV9

Insert

jGCaMP7f

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChR2(H134R)-GFP

Plasmid

#58880

Purpose

AAV expression of ChR2(H134R)-GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Has Service

AAV Retrograde and AAV8

Insert

ChR2(H134R)-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

H134R

Promoter

Syn

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-mEGFP

Plasmid

#175293

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mEGFP

Tags

mEGFP

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-GfaABC1D-Cre-4x6T

Plasmid

#196410

Purpose

Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Has Service

AAV5

Insert

Cre

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pY109 (lenti-LbCpf1)

Plasmid

#84740

Purpose

Lenti virus delivery of LbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
puromycin resistance gene

Use

Lentiviral

Tags

3xHA, NLS, and P2A

Promoter

EFS

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-15F11-HA-Halo

Plasmid

#129592

Purpose

The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.

Depositor

Tim Stasevich

Article

Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

Insert

Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-Cre-GFP

Plasmid

#68544

Purpose

AAV expression of Cre-GFP fusion

Depositor

Eric Nestler

Article

Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

Insert

Cre

Use

AAV and Cre/Lox

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only

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