We narrowed to 8,392 results for: crispr grnas

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pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only
non-targeting control gRNA (BRDN0001145269)

Plasmid

#80249

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pPN440

Plasmid

#137874

Purpose

PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN062

Plasmid

#91595

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN063

Plasmid

#91596

Purpose

Express sgRNA targeting human DFNA5

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

DFNA5 (GSDME Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC523

Plasmid

#62320

Purpose

sgRNA for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Expression

Yeast

Promoter

SNR52

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA1_NANOG

Plasmid

#64153

Purpose

Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1_NANOG (NANOG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pWT062d

Plasmid

#107911

Purpose

W2m.2-4 plasmid. Contains H1-TetR-sgRNA B and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

H1-TetR-sgRNA B

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062e

Plasmid

#107910

Purpose

W2m.2-3 plasmid. Contains U6-LacI-sgRNA A and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-LacI-sgRNA A

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-NEAT1m_v1

Plasmid

#97083

Purpose

Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

sgRNA NEAT1m_v1

Use

CRISPR

Promoter

U6

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMM710

Plasmid

#68893

Purpose

IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

Plasmid

#100716

Purpose

B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting ATP1A1 (cloned using BsmBI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_TIA1

Plasmid

#106097

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIA1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA TIA1

Use

CRISPR

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pgRNA-non-target

Plasmid

#129465

Purpose

Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Non-target/random gRNA

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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