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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,465 results for: 11

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Showing: 161 - 180 of 8465 results

  1. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Human, S. pyogenes, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71666-plasmid-map-sequence-id-129527

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  4. pAAV/D377Y-mPCSK9

    Plasmid
    #58376
    Purpose
    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis
    Depositor
    Jacob Bentzon
    Publication
    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do
    Has service
    AAV8
    Insert
    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    Use
    AAV
    Expression
    Mammalian
    Mutation
    D377Y
    Promoter
    HCRApoE/hAAT
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58376-plasmid-map-sequence-id-292482

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  11. pcDNA3-TORCAR(T/A)

    Plasmid
    #64928
    Purpose
    Negative-control mutant for TORCAR biosensor.
    Depositor
    Jin Zhang
    Publication
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TORCAR(T/A) (EIF4EBP1 Human, Synthetic)
    Tags
    Cerulean and YPet
    Expression
    Mammalian
    Mutation
    4EBP1 domain contains T37A and T46A mutations.
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64928-plasmid-map-sequence-id-109685

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  15. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

    Viral prep
    #62723-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato (Cre-dependent)
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 161 - 180 of 8465 results