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RNA-binding protein pooled CRISPR knockout library

Pooled library

#141438

Purpose

Knockout library targeting RNA-binding proteins with 10 guide RNAs per target.

Depositor

Eugene Yeo

Publication

Wheeler et al Nat Methods. 2020 May 11. pii: 10.1038/s

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Publication

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Publication

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

33 Articles

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LRG (Lenti_sgRNA_EFS_GFP)

Plasmid

#65656

Purpose

Lentiviral introduction of sgRNA constitute expression linked with GFP marker into mammalian cell line.

Depositor

Christopher Vakoc

Publication

Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter-driven sgRNA expression and EFS promo…

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

71 Articles

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FU-tet-o-hSox2

Plasmid

#19779

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Sox2 (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Publication

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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3544 pMIG Bcl-2

Plasmid

#8793

Depositor

Stanley Korsmeyer

Publication

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bcl-2 (BCL2 Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-TORCAR(T/A)

Plasmid

#64928

Purpose

Negative-control mutant for TORCAR biosensor.

Depositor

Jin Zhang

Publication

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TORCAR(T/A) (EIF4EBP1 Human, Synthetic)

Tags

Cerulean and YPet

Expression

Mammalian

Mutation

4EBP1 domain contains T37A and T46A mutations.

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET21-pelB-LaG-11

Plasmid

#172762

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Publication

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-11

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hNanogP8

Plasmid

#19776

Depositor

Konrad Hochedlinger

Publication

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

NanogP8 (NANOGP8 Human)

Use

Lentiviral

Expression

Mammalian

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAc5.1B-EGFP

Plasmid

#21181

Depositor

Elisa Izaurralde

Publication

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

enhanced green flourescent protein

Use

Drosophila expression

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pGL4 mIL-17 2kb promoter

Plasmid

#20124

Depositor

Warren Strober

Publication

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-Atg14L

Plasmid

#21635

Depositor

Tamotsu Yoshimori

Publication

Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Atg14L (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pminiTol2

Plasmid

#31829

Purpose

Vector with minimal Tol2 transposition sites, with capacity for delivering larger gene cargo than standard Tol2 vectors

Depositor

Stephen Ekker

Publication

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Type

Empty backbone

Use

Cloning vector

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCMV-HA-GASC1

Plasmid

#24214

Purpose

Mammalian expression of human JMJD2C with HA tag

Depositor

Kristian Helin

Publication

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

GASC1 (KDM4C Human)

Tags

HA

Expression

Mammalian

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMV-CRY2-mCherry

Plasmid

#58368

Purpose

Mammalian codon optimized CRY2

Depositor

Won Do Heo

Publication

Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2[1-498] (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (AAV5)

Viral prep

#62723-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] (#62723). In addition to the viral particles, you will also receive purified pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] plasmid DNA. Cre-dependent ChrimsonR-tdTomato under the control of the Synapsin promoter. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Promoter

Syn

Tags

tdTomato (Cre-dependent)

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only
pSG5-MAGE-A11

Plasmid

#89096

Purpose

mammalian expression of full-length human MAGE-A11

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAGE-A11 (MAGEA11 Human)

Expression

Mammalian

Promoter

SV40

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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