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Plasmid#112234PurposeHIVGKO contains a codon-switched eGFP under the control of the HIV-1 specific promoter, and mKO2 under the control of the constitutive promoter EF1aDepositorInsertcsGFP-EF1a-mKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dCas9
Plasmid#112195PurposeKRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertKRAB (ZNF10 Human)
UseCRISPRTags3XFLag-NLS-KRAB-dCas9-NLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX458-ECFP
Plasmid#112220PurposeCloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFPDepositorInsertECFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIGAhRC
Plasmid#112510Purposehuman Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoterDepositorUseSynthetic BiologyTagsExpressionYeastMutationPromoterGal1,10Available sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1a
Plasmid#112221Purposemammalian expression of flag tagged GREB1aDepositorInsertGREB1a (GREB1 Human)
UseTags3x flagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCathepsin S
Plasmid#11251PurposeMammalian expression of human Cathepsin SDepositorInsertcathepsin S (CTSS Human)
UseTagsC9 (GTETSQVAPA)ExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSAM_Bt
Plasmid#112497PurposeTransposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).DepositorInsertsINSeq-adapted mariner transposon
Himar1C9
UseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmIL-6promoterEGFP
Plasmid#112896PurposeTo monitor the activation of the murine IL-6 promoter by eGFP expressionDepositorInsertmurine IL-6 promoter (Il6 Mouse)
UseTagsExpressionMammalianMutationPromoterIL-6 promoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGExpressionMutationD10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag
Plasmid#112487PurposeMammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB
Plasmid#112225PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.DepositorInsertSuper-folder GFP
UseZebrafish expressionTagsExpressionMutationPromoterbeta actin 2 (zebrafish)Available sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-GFP
Plasmid#112851PurposeGeneral donor vector with T2A-H2B-GFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
UseTagsExpressionYeastMutationPromoter5 XREs+ minimal Cytochrome CAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_S94A-PolyA
Plasmid#112286PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Serine 94 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W
Plasmid#112926PurposeCRISPR-a reporter assay lentiviral vector (empty gRNA control)DepositorInserthU6-gRNASAM(empty), TREGFP, PGKpuroGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
RhoA sensor (LSS-SGFP2-mCherry)
Plasmid#112948PurposeRhoA FRET biosensorDepositorInsertRhoa sensor
UseTagsLSS-SGFP2 and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR26-CAG-H2B-miRFP703
Plasmid#112853PurposeDonor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell linesDepositorInsertH2B-miRFP703
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKC-4.04
Plasmid#112085PurposeControl for NMD sensor: pCMV-3XFLAG-Fluc-betaglobinDepositorInsert3XFLAG firefly luciferase-beta-globin (HBB Mouse, Human, Photinus pyralis)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5GP
Plasmid#112610PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GST and AviExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pExp-TF
Plasmid#112575PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-TriggerFactor and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only