We narrowed to 9,215 results for: pcas

1. pCAGGS_2paLINX

   Plasmid

   #129655

   Purpose

   2P-activatable light-inducible nuclear exporter

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paLINX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9_mouse_Adar1_ccB

   Plasmid

   #158122

   Purpose

   deletion mAdar1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (Adar Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9_mouse_Adar1_ccA

   Plasmid

   #158120

   Purpose

   deletion mAdar1

   Depositor

   Emanuela Felley-Bosco

   Insert

   Adar1 (Adar Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT222

   Plasmid

   #134890

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas5

   Plasmid

   #82378

   Purpose

   sgRNA targeting YFP expressed from pJ23119 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCasY1

   Plasmid

   #87687

   Purpose

   p15A plasmid expressing CasY1

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasY1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG:EYFP

   Plasmid

   #32596

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

   Insert

   EYFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCAGGs

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

8. HeFSpCas9

   Plasmid

   #92355

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity Cas9-NLS
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAGM9121_pCAT1

   Plasmid

   #160918

   Purpose

   Catalase 1 (CAT1) promoter module for episomal P. pastoris promoter shuffling

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al ACS Synth Biol. 2021 Jun 2. doi: 10.1021

   Insert

   Catalase 1 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAH01SpR

    Plasmid

    #128159

    Purpose

    pCAH01 derivative (Ptet inducible promoter) with ahp (kanamycin resistance) CDS replaced with the aadA (streptomycin/spectinomycin resistance) CDS

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. HeFm2SpCas9

    Plasmid

    #92111

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    “Highly enhanced Fidelity” mut2 SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, Q926A, R1060A

    Promoter

    Cbh

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAT221

    Plasmid

    #134889

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAM35

    Plasmid

    #86475

    Purpose

    Expresses p9-6His

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p9-6His
    

    Use

    Synthetic Biology

    Tags

    6His

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAD54_CAD29_H104A_pvp008

    Plasmid

    #124876

    Purpose

    Expresses hCAD His103Ala mutant in E.coli

    Depositor

    Konrad Buessow

    Article

    Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag and TEV site

    Expression

    Bacterial

    Mutation

    mutated His103 to Ala, deleted amino acids 1-3 an…

    Promoter

    T7

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCARSF85

    Plasmid

    #64694

    Purpose

    H6-TF-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TF

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS_2pabPAC

    Plasmid

    #158968

    Purpose

    2P-activatable bPAC for transient expression

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

    Insert

    2pabPAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA0.002

    Plasmid

    #119560

    Purpose

    Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

    Depositor

    Alistair McCormick

    Article

    Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas2F

    Plasmid

    #82377

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PEZ3

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. B-HeFSpCas9

    Plasmid

    #126766

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAS_gpyrG1

    Plasmid

    #90276

    Purpose

    (also pMST621-BB3_gpyrG1_cas9) CRISPR/Cas9 plasmid with gRNA for pyrG1, Cas9

    Depositor

    Michael Sauer

    Article

    Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

    Insert

    gRNA (pyrg1)
    

    Use

    A. niger

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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