We narrowed to 615 results for: vp64

1. pMSCV-LTR-dCas9-VP64-BFP

   Plasmid

   #46912

   Purpose

   Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   dCas9-VP64-BFP fusion
   Puromycin resistance
   

   Use

   CRISPR and Retroviral

   Tags

   3xNLS, BFP, and VP64 domain

   Expression

   Mammalian

   Promoter

   LTR and PGK

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEG302 22aa SunTag VP64 nog

   Plasmid

   #120251

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti Vp64-dCas9 T2A mTagBFP2

   Plasmid

   #171052

   Purpose

   Expression of Vp64-dCas9 activation system

   Depositor

   Agnel Sfeir

   Article

   Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

   Insert

   Vp64-dCas9 T2A mTagBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170995

   Purpose

   HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSBBi-Pur LaG17-synNotch-TetRVP64

   Plasmid

   #158133

   Purpose

   Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

   Depositor

   Neal Devaraj

   Insert

   LaG17-synNotch-TetRVP64
   

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJC-20xUAS-TALE3-VP64-P10

   Plasmid

   #104607

   Purpose

   Expresses TALE3 under UAS control

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   TALE3
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC-20xUAS-TALE4-VP64-P10

   Plasmid

   #104608

   Purpose

   Expresses TALE4 under UAS control

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   TALE4
   

   Use

   TALEN

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBBi-Pur LaG17-synNotch-Gal4VP64

    Plasmid

    #158135

    Purpose

    Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone

    Depositor

    Neal Devaraj

    Insert

    LaG17-synNotch-Gal4VP64
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTAL-MajSat-FLAG-NLS-VP64

    Plasmid

    #69074

    Purpose

    Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Expression

    Mammalian

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Promoter

    CMV/hUBC

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJC-repo1.9kb-TALE4-VP64-P10

    Plasmid

    #104612

    Purpose

    Expresses TALE4 under the control of a 1.9kb repo enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.9kb repo enhancer element (repo Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP304-pAAV-EFS-dSaCas9-VP64-pA

    Plasmid

    #113679

    Purpose

    A EFS driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC-J6-NbGP41-sfGFP-VP64-GB1-J9

    Plasmid

    #202007

    Purpose

    MoClo level 0 NbGP41-sfGFP-VP64-GB1 coding sequence module

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    NbGP41-sfGFP-VP64-GB1
    

    Use

    Synthetic Biology; Moclo cloning vector

    Tags

    GB1 and sfGFP

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. dSp-VP64-p65-RTA(75-190)

    Plasmid

    #99670

    Purpose

    Expresses dSp Cas9 fused to combination of truncated activation domains

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Tags

    VP64-p65-RTA(75-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    May 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEG302 22aa SunTag VP64 g4+g17

    Plasmid

    #120252

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEG302 5aa SunTag VP64 nog

    Plasmid

    #115480

    Purpose

    CRISPR-Cas9 SunTag system to target VP64 to specific loci of interest (nog=no guide)

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    NOS_NLS_GB1_NLS_linker_VP64_linker sfGFP_scFv_UBQ10_Insulator UBQ10_Ω dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJEP303-pAAV-CMV-dSaCas9-VP64-pA

    Plasmid

    #113678

    Purpose

    A CMV driven de-catalyzed SaCas9 fused to VP64 domain for increased transcription in targeted region

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    de-catalyzed SaCas9
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    NLS and VP64

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge