We narrowed to 5,041 results for: U6...

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T1_gRNA

Plasmid

#199387

Purpose

pDEL111; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type I sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type I sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T2_gRNA

Plasmid

#199388

Purpose

pDEL112; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type II sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type II sgRNA

Use

Tol2 destination vector

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_T3_gRNA

Plasmid

#199389

Purpose

pDEL113; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 type III sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 type III sgRNA

Use

Tol2 destination vector

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHACK-QF2-AgU6-mCherry

Plasmid

#184530

Purpose

HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

Depositor

Christopher Potter

Article

Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

Insert

QF2

Use

CRISPR

Promoter

Anopheles U6 promoter

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDest_U6_A2_CG2_typeEGF_gRNA

Plasmid

#199386

Purpose

pDEL110; Gateway Destination vector for cell-type specific CRISPR encoding nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Insert

nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_18bp

Plasmid

#174852

Purpose

Express forward pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_18bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_18bp

Plasmid

#174853

Purpose

Express reverse pegRNA mediating 991-bp deletion and 18-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_18bp

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegF_44bp

Plasmid

#174854

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_44bp

Plasmid

#174855

Purpose

Express forward pegRNA mediating 991-bp deletion and 44-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_44bp insertion

Expression

Mammalian

Promoter

U6

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-Mb-crRNA

Plasmid

#78959

Purpose

Cloning vector for expression of MbCpf1 crRNA. It contains BsmB1 site for cloning.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX459-sgAAVS1

Plasmid

#184403

Purpose

pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

AAVS1-specific sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJAT30

Plasmid

#204294

Purpose

For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-DsRed

Use

CRISPR

Promoter

MHC

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-G-Creon shRNA[Control]

Plasmid

#181824

Purpose

This vector simultaneously expresses GFP protein and shRNA in a Cre-dependent manner.

Depositor

Eun Mi Hwang

Article

Kim et al J Neurosci Methods. 2021 Dec 21:109452.

Type

Empty backbone

Use

AAV and RNAi

Expression

Mammalian

Promoter

EF1a/U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Blasto

Plasmid

#167904

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-MCS

Plasmid

#185594

Purpose

Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLX-sgRNA

Plasmid

#50662

Purpose

Lentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)

Depositor

Eric Lander, David Sabatini

Article

Wang et al Science. 2014 Jan 3;343(6166):80-4. doi:

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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AeU6 3p3 GFP LgRNA

Plasmid

#182208

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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