We narrowed to 18,123 results for: Met;
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Plasmid#238372PurposeEmpty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.DepositorInsertnmKate
UseTagsExpressionMutationPromotercmlc2 (myl7)Available sinceMay 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol1-Spe-KpnI-ubb-nmKate
Plasmid#238373PurposeEmpty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.DepositorInsertnmKate
UseTagsExpressionMutationPromoterubiquitinAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRetro-UMI
Plasmid#222686PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPGK without BbsI cutsitePromoterAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-NeoR-SV40 polyA-Z
Plasmid#218909Purposegolden gate cloningDepositorInsertmPGK-Neomycine-SV40 polyA
UseTagsExpressionMammalianMutation395T>CPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA332
Plasmid#214744PurposeLow copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-BlastR-Z
Plasmid#218907Purposegolden gate cloningDepositorInserthPGK-BlastR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-NeoR-Z
Plasmid#218906Purposegolden gate cloningDepositorInserthPGK-NeoR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-HygroR-Z
Plasmid#218905Purposegolden gate cloningDepositorInserthPGK-HygroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-BlastR-SV40 polyA-Z
Plasmid#218903Purposegolden gate cloningDepositorInsertmPGK-BlastR-SV40 polyA
UseTagsExpressionMammalianMutation2468T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
2x3APWWP-IFP2.0C
Plasmid#215752PurposeExpresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensorsDepositorInsert2x3APWWP-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
2xCBX7CD-IFP2.0C
Plasmid#215754PurposeExpresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensorsDepositorInsert2xCBX7CD-IFP2.0C
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2T_eGFP
Plasmid#208883PurposeExpress eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.DepositorInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations L191T and A225TPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2E_eGFP
Plasmid#208884PurposeExpress eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.DepositorInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations K192E and R233EPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCitro-15a
Plasmid#202033Purposep15a-based plasmid that encodes a conjugative system synthesized from metagenomic data for conjugation to CitrobacterDepositorInsertconjugative system from a Citrobacter spp.
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SUMO_LPETG_MT3
Plasmid#200304PurposeBacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavageDepositorInsertmetallothionein 3 (MT3 Human)
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10ΔF3
Plasmid#194857PurposeExpress EGFP-MYO10 with the F3 FERM domain lobes deleted (1-1951) in mammalian cells.DepositorInsertMYO10 ΔF3 (MYO10 Human)
UseTagsEGFPExpressionMammalianMutationTruncated MYO10 construct. MYO10 amino acids 1-1…PromoterCMVAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-RAPH1-F2
Plasmid#194864PurposeExpress the RAPH1 fragment (535-868) tagged to GST in bacteria.DepositorInsertRAPH1 1062-1250 (RAPH1 Human)
UseTagsGSTExpressionBacterialMutationamino acids 1062-1250PromoterTacAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F4
Plasmid#194863PurposeExpress the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.DepositorInsertRAPH1 (RAPH1 Human)
UseTagseGFPExpressionMammalianMutationamino acids 1062-1250PromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F3
Plasmid#194862PurposeExpress the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.DepositorInsertRAPH1 (RAPH1 Human)
UseTagseGFPExpressionMammalianMutationamino acids 868-1062PromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP-RAPH1-F2
Plasmid#194861PurposeExpress the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.DepositorInsertRAPH1 (RAPH1 Human)
UseTagseGFPExpressionMammalianMutationamino acids 535-868PromoterCMVAvailable sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_FGF21
Plasmid#182406PurposeBacterial expression of fgf21DepositorInsertfgf21 (FGF21 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen
Plasmid#182393PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusionsDepositorInsertsVNp-LZ
CydAB-mNeongreen
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed Free NES (M)
Plasmid#182437PurposeYeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsFPPS(M)
NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10 and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNHT7
Plasmid#186753PurposeA basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.DepositorTypeEmpty backboneUseSynthetic Biology; Cloning backboneTagsExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YF
Plasmid#171459PurposeExpresses YF, a FiCE of iCMM, in mammalian cellsDepositorInsertEYFP-FKBP12
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR-v1-sgNADK_1
Plasmid#163462Purposelentiviral vector expressing Cas9 and an sgRNA targeting NADKDepositorInsertsgRNA 1 targeting NADK (NADK Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-5U6-sgRNAs-hsyn-EGFP
Plasmid#112213PurposeTargeted DNA methylationDepositorInsertsgRNAs
UseAAVTagsExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-N-mCherry-C
Plasmid#159437PurposeCRISPR knock-in donor construct with fluorescent reporterDepositorInsertmCherry
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…ExpressionMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-574-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85875PurposeSensor PlasmidDepositorInsertmiR-574-3p Sensor (MIR574 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-345-5p.sensor.PGK.dNGFR.WPRE
Plasmid#85927PurposeSensor PlasmidDepositorInsertmiR-345-5p Sensor (MIR345 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-18b-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85896PurposeSensor PlasmidDepositorInsertmiR-18b-3p Sensor (MIR18B Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only