We narrowed to 41,961 results for: cas

1. p3s-Cas9-HN

   Plasmid

   #104171

   Purpose

   Cas9-WT mammalian expression

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

   Insert

   Cas9-WT
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Nov. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_KanR neo

   Plasmid

   #167869

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. TMPRSS2 g1 + Cas9

   Plasmid

   #153015

   Purpose

   A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + TMPRSS2 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   TagBFP2

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-Casp2

   Plasmid

   #50023

   Purpose

   pENTR vector for mouse Caspase-2

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   Casp2 (Casp2 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. RCAS-HA-b-actin

   Plasmid

   #125834

   Purpose

   Retroviral expression of HA-tagged human beta-actin

   Depositor

   Yi Li

   Article

   Toneff et al Neoplasia. 2010 Sep;12(9):718-26.

   Insert

   Beta-actin (ACTB Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC1300_pUB10_pco-vCASphi_E9t_MCS_version2

   Plasmid

   #197961

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pco-vCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1300_pUB10_pco-nCASphi_E9t_MCS_version2

   Plasmid

   #197962

   Purpose

   T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pco-nCasphi-2
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEF1a-nSpCas9-NG

   Plasmid

   #171692

   Purpose

   Expresses nSpCas9-NG in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9-NG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1a-nSpCas9

   Plasmid

   #171691

   Purpose

   Expresses nSpCas9 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

   Insert

   nSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   EF1a

   Available since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. NAX_CAGGS_CAS9_KanR neo

    Plasmid

    #167861

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_EE-nCas9

    Plasmid

    #163541

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    EE-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGC-I2Cas9

    Plasmid

    #173158

    Purpose

    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.

    Depositor

    Piotr Ziolkowski

    Article

    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:

    Inserts

    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Plant

    Mutation

    Contains a D169N mutation with no visible effect …

    Promoter

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AV44_pCAG.Cas9-D10A.gRNA.S1

    Plasmid

    #199258

    Purpose

    Expression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNA

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Inserts

    SpCas9-D10A nickase
    AAVS1-targeting gRNA
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter and RNA polymerase III promoter for …

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCascade-IS186

    Plasmid

    #140627

    Purpose

    Expresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS186. The crRNA-IS186 targets IS186 loci in Escherchia coli.

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS186
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cas12c-sgRNA_BbsI_CAM

    Plasmid

    #183221

    Purpose

    Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly

    Depositor

    Jennifer Doudna

    Article

    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

    Insert

    Cas12c single guide RNA containing BbsI sites in spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426_Cas9_gRNA-ARS106a

    Plasmid

    #87382

    Purpose

    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS106a sequence ATACGGTCAGGGTAGCGCCC in yeast chromosome 1.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS106a
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ADH1, pTyrosine

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9 (GB1079)

    Plasmid

    #75399

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. RCASBP-Y CHA

    Plasmid

    #11480

    Depositor

    William Pavan

    Article

    Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

    Insert

    Gateway ccdB reading frame cassette
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. RCAS-myc

    Plasmid

    #11548

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

20. AAEL003877-Cas9

    Plasmid

    #100581

    Purpose

    The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL003877-hspcas9-GFP
    Opie-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL003877 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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