We narrowed to 11,861 results for: Kars;

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SP-Cas9

Plasmid

#62731

Purpose

Expresses SP-Cas9 protein in bacterial cells

Depositor

Niels Geijsen

Article

D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

Insert

SP-CAS9

Use

CRISPR

Tags

His tag and NLS

Expression

Bacterial

Mutation

Codon optimazed for e.coli

Promoter

T7

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pETM6-Amp-T7-roGFP

Plasmid

#202464

Purpose

Expresses redox sensitive GFP (roGFP) with T7 induction

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Tags

6X-His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Tags

2xFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

Plasmid

#125695

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mScarlet-Gephyrin.FingR-IL2RGTC

Use

AAV

Expression

Mammalian

Promoter

EF1A

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDY1243-Fluorescent RADARS

Plasmid

#182540

Purpose

Express Fluorescent RADARS in bicistronic vector with mCherry as control gene

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

mNeon

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-UBC-gate-3xHA-pGK-PUR

Plasmid

#107393

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xHA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95.FingR-eGFP-CCR5TC

Plasmid

#125693

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti TurboRFP BlastR

Plasmid

#102343

Purpose

Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Article

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

TurboRFP

Use

Lentiviral

Tags

N/A

Promoter

CMV immediate early

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_mCherry_P2A_Hygro_Barcode

Plasmid

#120426

Purpose

Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

mCherry

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1c

Plasmid

#26802

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1c (SREBF1 Human)

Tags

2XFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDY1314_RADARS_Luciferase

Plasmid

#193191

Purpose

Express Gaussia Luciferase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

RADARS Luciferase

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pNuc-cis

Plasmid

#202796

Purpose

RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

Depositor

David Edgell

Article

Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

Insert

TevSpCas9, sgRNA cassette, oriT, CenArsHis

Expression

Bacterial

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-GFP-PAmin-GF3-R4A22

Plasmid

#203768

Purpose

For production of template RNA for use in PRINT, GFP under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Promoter

CMV

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-mH6-Cas12c1

Plasmid

#120872

Purpose

Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Cas12c1

Use

CRISPR

Tags

mH6

Expression

Bacterial

Mutation

WT

Promoter

lac

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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hSYN-mito-dsRED2

Plasmid

#173069

Purpose

mitochondria targeted dsRED2 expressed off of human Synapsin I promoter.

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

human synapsin 1

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-ZoAl-ENT-3xF-ORF

Plasmid

#238484

Purpose

For production of C-term flag-tagged ZoAl EN-tuned ORF mRNA for mRNA transfection in PRINT

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

R2 retroelement mRNA

Use

Ivt template

Tags

3x-Flag

Mutation

R1103A

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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Cp PS Intein tTA

Plasmid

#242026

Purpose

Blue-light-controlled release of tTA transactivator from the cytoplasm to the nucleus.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Cp PS Intein tTA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]

Plasmid

#247250

Purpose

Cloning backbone for measuring bridge recombinase mediated inversion

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1461_SERPINA1_CCA32_5avidity Cre

Plasmid

#193193

Purpose

Express Cre conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

Cre

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGFH-9

Plasmid

#39538

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pTW2154

Plasmid

#236578

Purpose

ISYmu1 CHLl1 gRNA4 TRV2 Architecture_B

Depositor

Steven Jacobsen

Article

Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

Insert

ISYmu1 TnpB

Expression

Plant

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mito-dsRED2

Plasmid

#173071

Purpose

mitochondria targeted dsRED2 expressed off of CAG promoter

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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