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173,361 results

Showing: 3681 - 3720 of 173361 results

  1. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available Since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  2. PBX1A-pCMV1

    Plasmid
    #21029
    Depositor
    Corey Largman
    Insert
    PBX1 (PBX1 Human)
    Expression
    Mammalian
    Available Since
    Aug. 14, 2009
    Availability
    Academic Institutions and Nonprofits only

  8. pOCC102

    Plasmid
    #118883
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118883-plasmid-map-sequence-id-232315

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  13. pAAV-mDlx-ChR2-mCherry-Fishell-3 (AAV1)

    Viral Prep
    #83898-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-mDlx-ChR2-mCherry-Fishell-3 (#83898). In addition to the viral particles, you will also receive purified pAAV-mDlx-ChR2-mCherry-Fishell-3 plasmid DNA. mDlx-driven expression of ChR2, fused to mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/
    Promoter
    mDlx
    Tags
    mCherry
    Available Since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only

  14. pD649-HAsp-PTPRF-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157036
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    PTPRF (PTPRF Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157036-plasmid-map-sequence-id-401022

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  18. p5E-elavl3

    Plasmid
    #75025
    Purpose
    Can be used to drive pan neuronal expression in zebrafish.
    Depositor
    Nicholas Cole
    Article
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    ELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
    Use
    5' entry vector for multisite gateway three …
    Promoter
    elavl3
    Available Since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75025-plasmid-map-sequence-id-141916

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  27. pGEX-6p1-GST-Ubiquitin

    Plasmid
    #223236
    Purpose
    Bacterial expression construct for GST-Tagged Ubiquitin production and purification
    Depositor
    Weixing Zhao
    Insert
    Ubiquitin
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Aug. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    223236-plasmid-map-sequence-id-443802

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  31. pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)

    Plasmid
    #72639
    Purpose
    Tol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domain
    Depositor
    Joshua Bonkowsky
    Insert
    UAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
    Use
    Gateway
    Mutation
    Stop codon after mCherry- SEE DEPOSITOR COMMENTS …
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72639-plasmid-map-sequence-id-429021

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  33. pAAV-CaMKIIa-hM3D(Gq)-mCherry (AAV8)

    Viral Prep
    #50476-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-hM3D(Gq)-mCherry (#50476). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hM3D(Gq)-mCherry plasmid DNA. CaMKIIa-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    mCherry
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  38. MSCV-FoxP3-IRES-Thy1.1

    Plasmid
    #229616
    Purpose
    Mammalian expression of full length mouse FoxP3
    Depositor
    Sun Hur
    Insert
    full length FoxP3 (Foxp3 Mouse)
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229616-plasmid-map-sequence-id-457336

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  39. pGEX-2T-Rac1-T17N

    Plasmid
    #12978
    Depositor
    Gary Bokoch
    Insert
    Rac1 DN (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Available Since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  40. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid
    #233371
    Purpose
    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038
    Inserts
    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    Use
    Lentiviral
    Tags
    FLAG, NES, and mEGFP
    Promoter
    EF1α
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    233371-plasmid-map-sequence-id-466776

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Showing: 3681 - 3720 of 173361 results