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TU#708

Plasmid

#15277

Depositor

Martin Chalfie

Article

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

czgfp

Expression

Worm

Mutation

leucine zipper fused to c-terminal half of gfp

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223-AKT3

Plasmid

#23539

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway Cloning

Mutation

S2R

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDEST/N1-hEB1-GFP

Plasmid

#27382

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA HA FAK Y861F

Plasmid

#50508

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y861F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_MPC2

Plasmid

#131930

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MPC2 (MPC2 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_MFSD8

Plasmid

#131882

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD8 (MFSD8 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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hSyn Marina-T2A-nls-mCherry

Plasmid

#85843

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

GEVI Marina

Tags

mCherry

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; super ecliptic pH…

Promoter

hSyn1

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 A775_G776insV, G776C

Plasmid

#40979

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A775_G776insV, G776C

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
BCR/ABL P190 transgenic construct

Plasmid

#38185

Depositor

Nora Heisterkamp

Article

Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Use

Construct for making transgenic mice

Promoter

truncated mouse MT promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX1 myr Akt1 delta4-129

Plasmid

#15989

Depositor

Morris Birnbaum

Article

Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

Insert

Akt1 (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Delta 4-129

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
yfpsyndat

Plasmid

#19991

Depositor

Jonathan Javitch

Article

Carvelli et al J Neurochem. 2002 May . 81(4):859-69.

Insert

yfpsynhdat (SLC6A3 Human)

Tags

yfp

Expression

Mammalian

Available Since

Sept. 25, 2009

Availability

Academic Institutions and Nonprofits only

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pLpA-DN.JNK1

Plasmid

#51942

Purpose

Adenoviral vector for dominant negative JNK1 expression

Depositor

Jeffery Molkentin

Article

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK1 (Mapk8 Mouse)

Use

Adenoviral

Tags

FLAG

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-TGFBR2

Plasmid

#23623

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TGFBR2 (TGFBR2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3 GRK2 S670D

Plasmid

#45756

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 S670D (GRK2 Bovine)

Expression

Mammalian

Mutation

S670D

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PIK3R5

Plasmid

#20576

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIK3R5 (PIK3R5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDKN1B

Plasmid

#20420

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDKN1B (CDKN1B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SspB-mScarlet-I

Plasmid

#178521

Purpose

Expresses SspB-mScarlet-I in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

SspB-mScarlet-I

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC15A4

Plasmid

#131910

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC15A4 (SLC15A4 Human)

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
NRAS

Plasmid

#55654

Purpose

expression of mouse NRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NRAS (Nras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-INPP5K

Plasmid

#171950

Purpose

Expresses INPP5K in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP5K (INPP5K Human)

Tags

mCherry

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNT

Plasmid

#138681

Purpose

Expresses a non-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNT

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC34A2

Plasmid

#132088

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC34A2 (SLC34A2 Human)

Expression

Mammalian

Available Since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-TIE1

Plasmid

#23946

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TIE1 (TIE1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pPS2966

Plasmid

#8925

Depositor

Pamela Silver

Article

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Insert

NDR1 (STK38 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
GFP-PKCA WT

Plasmid

#195202

Purpose

Mammalian expression vector for GFP tagged PKCA.

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL(CMV)GLuc/CMV/WPREdU3

Plasmid

#136999

Purpose

Reporter vector encoding Gaussia luciferase

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Gaussia luciferase

Use

Lentiviral

Promoter

CMV

Available Since

Feb. 7, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221_SLC11A2

Plasmid

#131884

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC11A2 (SLC11A2 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONOR-tagBFP-PSM-dTOMATO

Plasmid

#100604

Purpose

Donor scafold with dTOMATO positive selection module, and tagBFP negative selection module

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Type

Empty backbone

Use

Synthetic Biology

Tags

dTOMATO-T2A-Puro and tagBFP

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 Flag p38 alpha(agf)

Plasmid

#20787

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (agf) (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

replaced Thr180 and Tyr182 with Ala and Phe, resp…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
NpT7-5-Erk2

Plasmid

#39230

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

Erk2 (Mapk1 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pCDFDuet-MKK6-EE p38alpha

Plasmid

#82719

Purpose

Expresses constitutively active human MKK6 and murine p38 alpha.

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Inserts

MKK6 (MAP2K6 Human)
p38alpha (Mapk14 Mouse)

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pShuttle-EGFP-CLASP2 340-1362

Plasmid

#24372

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (340-1362) (CLASP2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

CLASP2 deletion mutant that retains MT lattice bi…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pYFP-NGAT(A193T,N194Y)

Plasmid

#11388

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Changed Alanine 193 to Threonine, and Asparagine …

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac GST-SAS6

Plasmid

#80273

Purpose

baculovirus creation plasmid for GST-SAS6

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

SAS6 (SASS6 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTXB3-Flag PLK1

Plasmid

#68271

Purpose

bacterial expression of Plk1

Depositor

Stefano Ferrari

Insert

PLK1 (PLK1 Human)

Tags

Flag and Mxe intein/chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pLNCX1 HA AKT2

Plasmid

#60129

Purpose

expresses HA tagged AKT2

Depositor

Morris Birnbaum

Insert

protein kinase B (AKT2 Human)

Use

Retroviral

Tags

HA

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
RhoA

Plasmid

#55699

Purpose

expression of mouse RhoA

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoA (Rhoa Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe(SV40)-Cytochrome C-GFP

Plasmid

#41184

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C-GFP

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
FUW TetO HA-AMPKa2 WT

Plasmid

#69826

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-LacR-TopBP1

Plasmid

#31313

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
p2xFlag CMV2-YAP2-deltaC

Plasmid

#21123

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

5 Carboxyterminal amino acids -FLTWL are deleted

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-FES

Plasmid

#23876

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FES (FES Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK7a1 (K76A)

Plasmid

#14541

Depositor

Roger Davis

Article

Tournier et al Proc Natl Acad Sci U S A. 1997 Jul 8. 94

Insert

MKK7a1(K76A) (Map2k7 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Lys-76Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HK3

Plasmid

#20414

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HK3 (HK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CDK4

Plasmid

#20448

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC6A8

Plasmid

#131915

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A8 (SLC6A8 Human)

Expression

Mammalian

Available Since

Oct. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge

Showing: 4751 - 4800 of 15769 results

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