Addgene

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pKLV2-EF1a-ArgSen (-)NLS

Plasmid

#184689

Purpose

Lentiviral transfer plasmid for expression of ArgSen (-)NLS

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen (-)NLS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV2-EF1a-ArgSen

Plasmid

#184691

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-ArgSen

Plasmid

#184700

Purpose

Lentiviral transfer plasmid for expression of ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

ArgSen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

Plasmid

#157674

Purpose

Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

SpyCatcher with ddFLN4, ELP, Histag and ybbR tag

Use

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod(XL2V)-Doc

Plasmid

#157676

Purpose

Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc mutant XL2V with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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1xHRE-MinTK-CRE

Plasmid

#141142

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system.

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

1xHRE-MinTK-CRE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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3xHRE-MinTK-CRE

Plasmid

#141143

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

3xHRE-MinTK-CRE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMMLV-EGFP-P2A-Puro

Plasmid

#160226

Purpose

Expresses EGFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EGFP-P2A-PuroR

Use

Retroviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

LTR

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pMMLV-mRFP1-P2A-Puro

Plasmid

#160228

Purpose

Expresses mRFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

mRFP1-P2A-Puro

Use

Retroviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

LTR

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(CAT)T7-SB100

Plasmid

#34879

Depositor

Zsuzsanna Izsvak

Article

Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

Insert

SB100X transposase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-Ngn3ER-PuroR-mCherry

Plasmid

#197385

Purpose

To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

Ngn3ER-2A-PuroR-2A-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Tags

Expression

Mutation

contains an altered Cre gene modified by the addi…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP ArgSen

Plasmid

#184704

Purpose

Lentiviral transfer plasmid for expression of GFP ArgSen

Depositor

Brian Van Tine

Article

Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

Insert

GFP ArgSen

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Hyg-RFPKDEL

Plasmid

#138660

Purpose

Reporter plasmid expressing the ER-located RFP

Depositor

Bertrand Collet

Article

Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

Insert

RFP-KDEL

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGW1-Myc-DJ1-WT

Plasmid

#29347

Depositor

Mark Cookson

Article

Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

Insert

DJ1 (PARK7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC EJ7-GFP

Plasmid

#113617

Purpose

Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

EJ7-GFP variant of EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Article

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Use

Tags

His-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only