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We narrowed to 5,133 results for: puc

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Showing: 4851 - 4900 of 5133 results

  22. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  28. qTAG-C-mStayGold-EFS-Blast-ARL13B

    Plasmid
    #227278
    Purpose
    Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ARL13B Homology Arms flanking a mStayGold-EFS-Blast Cassette (ARL13B Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227278-plasmid-map-sequence-id-451732

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  29. qTAG-C-moxGFP-Puro-LAMP1

    Plasmid
    #207788
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a moxGFP-Puro Cassette (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207788-plasmid-map-sequence-id-416383

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  30. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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  31. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  34. qTAG-C-Solo-mStayGold-COX8A

    Plasmid
    #227309
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227309-plasmid-map-sequence-id-451688

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  38. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

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  40. qTAG-C-Solo-mStayGold-LAMP1

    Plasmid
    #227322
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LAMP1 Homology Arms flanking a mStayGold Tag (LAMP1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227322-plasmid-map-sequence-id-452101

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  41. qTAG-N-Solo-mScarlet-LMNB1

    Plasmid
    #207771
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a mScarlet Tag (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207771-plasmid-map-sequence-id-416246

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  42. qTAG-N-Puro-mStayGold-LMNB1

    Plasmid
    #227329
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227329-plasmid-map-sequence-id-451729

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  44. qTAG-N-Puro-mStayGold-ACTB

    Plasmid
    #227320
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-2A-mStayGold Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227320-plasmid-map-sequence-id-452091

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  45. qTAG-N-Blast-dTAG-LMNB1

    Plasmid
    #207776
    Purpose
    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207776-plasmid-map-sequence-id-416195

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  46. qTAG-N-Solo-mStayGold-ACTB

    Plasmid
    #227326
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mStayGold Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227326-plasmid-map-sequence-id-452114

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  48. qTAG-N-Blast-mScarlet-ACTB

    Plasmid
    #207753
    Purpose
    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Dec. 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207753-plasmid-map-sequence-id-416363

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  49. qTAG-N-Solo-mStayGold-CEP135

    Plasmid
    #227287
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP135 Homology Arms flanking a mStayGold Tag (CEP135 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227287-plasmid-map-sequence-id-452057

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Showing: 4851 - 4900 of 5133 results