We narrowed to 15,055 results for: UTY

1. pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

   Plasmid

   #45574

   Purpose

   Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   co I-Sce I
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

   Plasmid

   #85425

   Purpose

   Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   anti-Her2_4D5-8_synNotch
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-C1-TFIIB

   Plasmid

   #26675

   Depositor

   Sui Huang

   Article

   Chen et al Mol Biol Cell. 2002 Jan . 13(1):276-84.

   Insert

   TFIIB (GTF2B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pBT2dR3-proB

   Plasmid

   #122595

   Purpose

   BE PACE selection, express T7 RNA polymerase with degron-GTCCA target

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   T7 RNA polymerase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC-AdEvolve-DEST

   Plasmid

   #131692

   Purpose

   A version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.

   Depositor

   Matthew D. Shoulders

   Article

   Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

   Type

   Empty backbone

   Use

   Adenoviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTfR-PAmKate

   Plasmid

   #32692

   Depositor

   Vladislav Verkhusha

   Article

   Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

   Insert

   PAmKate
   

   Use

   Tags

   transferrin receptor (TfR)

   Expression

   Mammalian

   Mutation

   F84W/S148N/S165G compared to mKate (but numbering…

   Promoter

   CMV

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p3s-Sniper-Cas9

   Plasmid

   #113912

   Purpose

   Expresses Sniper-Cas9 with CMV promoter

   Depositor

   Jungjoon Lee

   Article

   Lee et al Nat Commun. 2018 Aug 6;9(1):3048. doi: 1

   Insert

   Sniper-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCali2

   Plasmid

   #86119

   Purpose

   Multiplex temperature-gated expression of mWasabi and mCherry reporters

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA36
   mWasabi
   TcI
   mCherry
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A67T and D341A, P60L, D135V, K187R, K202I, L208Q

   Promoter

   pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR_PGK_antiHer24D5-5_synNotch_Gal4VP64

   Plasmid

   #85423

   Purpose

   Lentiviral vector for expression of anti-Her2 scFv 24D5-5 Gal4VP64 synthetic Notch

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   anti-Her2_4D5-5 synNotch
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   PGK

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmTurquoise2-NES

    Plasmid

    #36206

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    NES
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBT120b

    Plasmid

    #122604

    Purpose

    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    gIII, luxAB, guide RNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBT44c

    Plasmid

    #122600

    Purpose

    BE PACE selection, express C-intein-Cas9-ugi fusion

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    intein-dCas9-ugi fusion
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKL003

    Plasmid

    #98919

    Purpose

    Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Insert

    PROPS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23118 - Strong Constitutive

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FU-tet-o-hSox2

    Plasmid

    #19779

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. H2B-pDendra2(N)

    Plasmid

    #75283

    Purpose

    Expresses H2B-Dendra2 in mammalian cells

    Depositor

    Xavier Darzacq

    Article

    Recamier et al Nucleus. 2014 Jan-Feb;5(1):75-84. doi: 1

    Insert

    H2bk (H2BC12 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Dronpa-Lifeact-7

    Plasmid

    #54740

    Purpose

    Localization: Actin, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

    Type

    Empty backbone

    Use

    Tags

    Dronpa-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

    Plasmid

    #64113

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRF0G-IodoY

    Plasmid

    #113645

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    IodoYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge