We narrowed to 15,118 results for: pET

1. pET30a-GlmU-NahK-ET64

   Plasmid

   #234663

   Purpose

   Expression an ELP-tagged fusion enzyme of GlmU and NahK

   Depositor

   Xianwei Liu

   Article

   Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

   Inserts

   N-acetylglucosamine- 1-phosphate uridyltransferase
   N-acetylhexosamine 1-kinase
   

   Tags

   ELP

   Expression

   Bacterial

   Available Since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-Cas9-NLS-6xHis

   Plasmid

   #62933

   Purpose

   Expression of Cas9-NLS-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9-NLS-6xHis
   

   Tags

   6xHis tag and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24a-VHH-tev-mCherry

   Plasmid

   #117752

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP, tev cleavage site and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al J Vis Exp. 2019 Feb 21;(144). doi: 10.37

   Insert

   anti-GFP nanobody fused to a T7, tev site, mCherry, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, and T7

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET151-GST-PP-RPN13 (19-132)

   Plasmid

   #73742

   Purpose

   Expression of human RPN13 PRU domain in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN13 (ADRM1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   PRU domain only (residues 19-132)

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pMG046 pETARA-CK1α-His

   Plasmid

   #154074

   Purpose

   Expresses GST_CK1α (human CK1α as a fusion protein with GST and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   CSNK1A1 (CSNK1A1 Human)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28b-RfxCas13d-His

   Plasmid

   #141322

   Purpose

   Plasmid for bacterial expression and purification of RfxCas13d protein

   Depositor

   Miguel Angel Moreno-Mateos, Ariel Bazzini

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b-ULP1-405-621

   Plasmid

   #229224

   Purpose

   For expression of ULP1 protein

   Depositor

   Andy LiWang

   Insert

   ULP1 (ULP1 Budding Yeast)
   

   Expression

   Bacterial

   Mutation

   405-621 (truncated)

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-UbcH5b/2419

   Plasmid

   #11475

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5b (UBE2D2 Human)
   

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Tags

   His x10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28sumo-hRig-I-Full-length

    Plasmid

    #210827

    Purpose

    protein expression in ecoli

    Depositor

    Anna Pyle

    Article

    Luo et al Structure. 2012 Nov 7;20(11):1983-8. doi

    Insert

    hRIG-I (DDX58) Full length protein (RIGI Human)
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-6XHis-SUMO-SIRT2

    Plasmid

    #102622

    Purpose

    Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Tags

    6XHis-SUMO

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-37 and 357-389

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-6xHis-(pos36)GFP

    Plasmid

    #62937

    Purpose

    Expression of 6xHis-(+36)GFP in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (pos36)GFP
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Kathleen Burns, Liam Holt

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid

    #107292

    Purpose

    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

    Depositor

    Dmytro Yushchenko

    Article

    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

    Insert

    SNCA-GFP (SNCA Human)
    

    Tags

    His-tag and SUMO-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a SnoopTag-mEGFP-SpyTag

    Plasmid

    #72325

    Purpose

    Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-mEGFP-SpyTag
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZH81 pET21b Rat1-His

    Plasmid

    #231650

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rat1 with a C-terminal his tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    Rat1 (RAT1 Budding Yeast)
    

    Tags

    6His

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-His-SCov2-nsp7

    Plasmid

    #154757

    Purpose

    Expression of SARS-CoV-2 nsp7

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-His-SCov2-nsp8

    Plasmid

    #154758

    Purpose

    Expression of SARS-CoV-2 nsp8

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-NlaCascade-NLS-6xHis

    Plasmid

    #180213

    Purpose

    Expressing Neisseria lactamica Type I-C Cascade subunits (Cas5-Cas8-Cas11-Cas7) with NLS and His tag on the C terminus of Cas7 for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas5-Cas8-Cas7
    

    Tags

    6XHis and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET-PE2-His (IK1822)

    Plasmid

    #170103

    Purpose

    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS

    Depositor

    Keith Joung

    Article

    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103

    Insert

    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    

    Use

    CRISPR

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Bacteria codon-optimized nSpCas9 (H840A) & M-…

    Promoter

    T7

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET-21a(+)-IPP1-His

    Plasmid

    #118978

    Purpose

    Bacterial expression plasmid for Inorganic pyrophosphatase

    Depositor

    Sebastian Maerkl

    Article

    Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

    Insert

    inorganic diphosphatase IPP1 (IPP1 Budding Yeast)
    

    Tags

    6x-His

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-15b-TEV-C9R

    Plasmid

    #118754

    Purpose

    TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

    Depositor

    Yutaka Kuroda

    Article

    Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

    Insert

    Tobacco Etch Virus protease (NEWENTRY Synthetic)
    

    Tags

    C9R and His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. PET-eSpCas9-NLS-6xHis

    Plasmid

    #207379

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET-51b(+)_NAD-Snifit

    Plasmid

    #113658

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NAD-Snifit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET-29b(+)_NSP4ss_SerM_NucA-myc

    Plasmid

    #218191

    Purpose

    Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

    Depositor

    Florence Pojer

    Insert

    nucA
    

    Tags

    NSP4 signal secretion peptide and myc

    Expression

    Bacterial

    Mutation

    codon optimized ecoli residues - 22-266

    Promoter

    T7

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. pET-51b(+)_NADP-Snifit

    Plasmid

    #113913

    Purpose

    Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

    Depositor

    Kai Johnsson

    Article

    Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

    Insert

    NADP-Snifit
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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