Showing: 81 - 100 of 2424 results
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Plasmid#169940PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and tBFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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pMax_BFP_A191G
Plasmid#177823PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).DepositorInserttagBFP_A191G
UseTagsExpressionMammalianMutationA191GPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMax_ScaI_BFP
Plasmid#177824PurposeSite-specific mutagenesis of tagBFP for the purposes of creating a clean double strand break for the purposes of measuring NHEJ activity via Host Cell Reactivation (HCR).DepositorInserttagBFP
UseTagsExpressionMammalianMutation5' inserted ScaI RE cut sitePromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_EBFP_Donor
Plasmid#178089PurposeHDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.DepositorInsertEBFP (T65S/F145Y)
UseCRISPRTagsExpressionMammalianMutationPromoterHuman PGK1AvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G3_EBFP_Nick_Dual_sgRNA
Plasmid#178094PurposeControl vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.DepositorInsertATP1A1 G3 + EBFP nick sgRNAs
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA
Plasmid#178100PurposeControl vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.DepositorInsertATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
UseCRISPR; Prime editingTagsExpressionMammalianMutationPromoterTandem U6 promotersAvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianMutationPromoterhuman EF1alphaAvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_TagBFP
Plasmid#113724PurposeExpresses the fluorescent protein TagBFP in a lentiviral backboneDepositorInsertTagBFP
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mTagBFP2
Plasmid#113725PurposeExpresses the fluorescent protein mTagBFP2 in a lentiviral backboneDepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_dectintagBFP2_WPRE
Plasmid#91786PurposeConstitutive expression of mTagBFP2-Dectin1A-N-10 using PGK promoter in a lentiviral backbone.DepositorInsertDectin1A (CLEC7A Human)
UseLentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
pRRlsinPGK_BFP2_WPRE
Plasmid#91787PurposeConstitutive expression of mTagBFP2 using PGK promoter in a lentiviral backbone.DepositorInsertmTagBFP2
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailabilityAcademic Institutions and Nonprofits only -
ATP1A1_T804N_hPGK1_EBFP_Donor
Plasmid#187455PurposeHDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.DepositorInsertATP1A1-T804N EBFP donor (ATP1A1 Human)
UseTagsExpressionMammalianMutationPromoterhPGK1AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_tBFP_PGK_mCitrine
Plasmid#188386PurposeLentiviral vector - tBFP reporter for Gal4-based SNIPRs with a constitutive mCitrine;DepositorInsertGal4UAS_tBFP_PGK_mCitrine
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_LuxSit_i_mTagBFP2
Plasmid#194300PurposeMammalian expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsmTagBFP2ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSExpressionMutationE174R, S542R, K548RPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSA_0_mTagBFP2_synCoTC
Plasmid#199472PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mTagBFP2_synCoTC
Plasmid#199473PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2_synCoTC
Plasmid#199474PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKozak_mTagBFP2_synCoTC
Plasmid#199493PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 2424 results