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Addgene
Showing: 41 - 60 of 576 results

  7. pJK294

    Plasmid
    #73373
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)
    Depositor
    T. J. Kappock
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changed histidine-59 to aspartate and histidine-8…
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    73373-plasmid-map-sequence-id-135109

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  13. PMXS-SLC1A3

    Plasmid
    #72873
    Purpose
    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly
    Depositor
    David Sabatini
    Article
    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10
    Insert
    SLC1A3 (SLC1A3 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-DCX(S306D)-2A-mCherry

    Plasmid
    #118292
    Purpose
    Expresses human mutant DCX tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Serine at position 306 substituted for aspartic acid
    Depositor
    Vance Lemmon
    Article
    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5
    Insert
    Doublecortin (DCX Human)
    Use
    AAV
    Tags
    2A peptide and mCherry
    Expression
    Mammalian
    Mutation
    Serine 306 changed to Aspartic Acid
    Promoter
    CMV with beta global intron
    Availability
    Academic Institutions and Nonprofits only
    118292-plasmid-map-sequence-id-229506

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  16. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid
    #122681
    Purpose
    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)
    Depositor
    Liming Pei
    Article
    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:
    Insert
    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Aspartic Acid 153 to Alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    122681-plasmid-map-sequence-id-244746

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  17. pTwist-SFFV-NFE2L18ND-Puro

    Plasmid
    #181918
    Purpose
    Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.
    Depositor
    Scott Dixon
    Article
    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11
    Insert
    NFE2L1 (NFE2L1 Human)
    Use
    Lentiviral
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    8 putative N-glycoyslation sites converted from a…
    Promoter
    SFFV
    Availability
    Academic Institutions and Nonprofits only
    181918-plasmid-map-sequence-id-358683

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Showing: 41 - 60 of 576 results