Addgene

Showing: 21 - 40 of 42 results

Results per page:

Sort results by:

B95(DE3) ΔA ΔfabR ΔserB

Bacterial Strain

#197655

Purpose

This strain is used for expressing phosphoserine-containing proteins using genetic code expansion without buildup of prematurely truncated protein.

Depositor

Richard Cooley, Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Bacterial Resistance

None

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only
Escherichia coli DH10B-MOB

Bacterial Strain

#229395

Purpose

Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Bacterial Resistance

60 ug/ml of dap

Species

Escherichia coli

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only
MK01

Bacterial Strain

#195090

Purpose

Improved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).

Depositor

Sander Tans

Article

Kogenaru et al J Biol Eng. 2014 Jan 2;8(1):2. doi: 10.1

Bacterial Resistance

Chloramphenicol

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only
TP997

Bacterial Strain

#13055

Depositor

Anthony Poteete

Article

Poteete et al BioTechniques. 2006 Sept. 41(3):261-264

Bacterial Resistance

Ampicillin and spectinomycin

Species

Tn1696

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
UQ6148

Bacterial Strain

#37178

Depositor

Gary Roberts

Bacterial Resistance

Ampicillin and kanamycin

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
RHO3

Bacterial Strain

#124700

Purpose

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Depositor

Herbert Schweizer

Article

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

Bacterial Resistance

Dap (400 µg/ml)

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
Strain SIJ_488

Bacterial Strain

#68246

Purpose

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.

Depositor

Alex Nielsen

Article

Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

Bacterial Resistance

None

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only
CJW4617

Bacterial Strain

#177118

Purpose

E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

Depositor

Christine Jacobs-Wagner

Article

Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

Bacterial Resistance

Kanamycin

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
KJ740(DE3)

Bacterial Strain

#179486

Purpose

KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Bacterial Resistance

Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

Species

Escherichia coli

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Jan Kok, Todd Klaenhammer

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
HL 6845

Bacterial Strain

#69785

Purpose

MC4100 + agn43 coding sequence replaced by T7RNAP and camR + Δdam + ΔSgrS

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Bacterial Resistance

Chloramphenicol

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
HL 6846

Bacterial Strain

#69786

Purpose

MC4100 + agn43 coding sequence replaced by T7RNAP and camR + ΔOxyR + ΔSgrS

Depositor

Han Lim

Article

Shin et al Nucleic Acids Res. 2016 Apr 15. pii: gkw

Bacterial Resistance

Chloramphenicol

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
EcNR2 Strain

Bacterial Strain

#26931

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Chloramphenicol and ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
HL 4195

Bacterial Strain

#52943

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(partial)

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HL 6139

Bacterial Strain

#52946

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(stop codon)

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HL 6279

Bacterial Strain

#52949

Purpose

MG1655 + fimS (off orientation)::gfpAAV tag::Asp terminator::CamR at fimA + ΔlacI + KanR replaces fimB and fimE(partial)

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
HL 6166

Bacterial Strain

#52947

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator at fimA + ΔlacI + KanR replaces fimB and fimE(IRL)

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Kanamycin

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
Akaby

Bacterial Strain

#184224

Purpose

Akaby is a RecB knockout E. coli strain, made for cell-free expression system for linear templates.

Depositor

Kate Adamala

Article

Sato et al PLoS One. 2022 Apr 7;17(4):e0266272. doi

Bacterial Resistance

Kanamycin

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only
DH10beta F' DOT sbcC (donor strain)

Bacterial Strain

#12082

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Bacterial Resistance

Tetracycline

Available since

March 1, 2007

Availability

Academic Institutions and Nonprofits only
rEcoli XpS

Bacterial Strain

#192872

Purpose

MG1655-C321 -- All 321 UAG codons changed to UAA

Depositor

Jesse Rinehart

Article

Gassaway et al Nat Methods. 2022 Oct 24. pii: 10.1038/s

Bacterial Resistance

Bleocin (zeocin)

Species

Escherichia coli

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Showing: 21 - 40 of 42 results

Results per page:

Sort results by:

B95(DE3) ΔA ΔfabR ΔserB

Escherichia coli DH10B-MOB

MK01

TP997

UQ6148

RHO3

Strain SIJ_488

CJW4617

KJ740(DE3)

EC1000

HL 6845

HL 6846

EcNR2 Strain

HL 4195

HL 6139

HL 6279

HL 6166

Akaby

DH10beta F' DOT sbcC (donor strain)

rEcoli XpS

Help Center

Deposit Plasmids

General Reagents

Viral Service

DNA Service

Plasmid Collections

	More than 20 requests
	More than 50 requests
	More than 100 requests