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Fucci(CA) hCdt1-iRFP hGeminin-iRFP

Plasmid

#190182

Purpose

Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
iRFP
hGeminin(1/110)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSF3-microID

Plasmid

#172880

Purpose

expresses microID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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HA-VHL-pRc/CMV

Plasmid

#19999

Depositor

William Kaelin

Article

Iliopoulos et al Nat Med. 1995 Aug . 1(8):822-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wild type

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-EGFP

Plasmid

#65264

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pNA0306

Plasmid

#169452

Purpose

pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Cas9

Expression

Yeast

Promoter

TEF1p

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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sAB-K29

Plasmid

#204735

Purpose

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Depositor

Minglei Zhao

Article

Yu et al Nat Chem Biol. 2021 Aug;17(8):896-905. d

Insert

synthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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BCR/ABL P190 transgenic construct

Plasmid

#38185

Depositor

Nora Heisterkamp

Article

Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

Insert

BCR/ABL P190 (ABL1, BCR Human)

Use

Construct for making transgenic mice

Promoter

truncated mouse MT promoter

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Article

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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Green Lindoblum

Plasmid

#163113

Purpose

Expresses biosensor for lactate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Lindolum

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLG009 (DRT type 1)

Plasmid

#157887

Purpose

Expresses the DRT type 1 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 1 (RT-nitrilase)

Expression

Bacterial

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP

Plasmid

#21181

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

enhanced green flourescent protein

Use

Drosophila expression

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING V155M

Plasmid

#102591

Purpose

Lentivector to express STING V155M and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-WT

Plasmid

#133749

Purpose

Wild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimd

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Wild type EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-3xFLAG-HIS3MX6

Plasmid

#20753

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP46

Plasmid

#22584

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP46 (USP46 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso1

Plasmid

#159585

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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pH-nCas9-PPE-V2

Plasmid

#170131

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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HRE-oUnaG

Plasmid

#201712

Purpose

Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Expression

Mammalian

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO SwiChRca-TS-EYFP

Plasmid

#55631

Purpose

Cre-Dependent Chloride Channel

Depositor

Karl Deisseroth

Article

Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

Insert

iC1C2-TS-EYFP

Use

AAV and Cre/Lox

Tags

EYFP

Expression

Mammalian

Mutation

C128A

Promoter

Ef1a

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-DYRK1B

Plasmid

#23761

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DYRK1B (DYRK1B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-Hs beta1

Plasmid

#45927

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Hs Beta1 adaptin (AP1B1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pUCP30T-eCFP

Plasmid

#78476

Purpose

Broad range bacterial fluorescence labeling (ECFP)

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eCFP

Expression

Bacterial

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-OSH2-2xPH

Plasmid

#161987

Purpose

Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

Insert

OSH2 (OSH2 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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Enterovirus D68 (EV-D68) strain EV-D68 STL 2014 12 3C protease

Plasmid

#228644

Purpose

Bacterial expression of the D68 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus D68 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-IGSF8-COMP5AP-AviTag-9xHis

Plasmid

#157476

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

IGSF8 (IGSF8 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLJC6-EV

Plasmid

#163454

Purpose

empty vector control for pLJC6

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pLG011 (DRT type 3)

Plasmid

#157889

Purpose

Expresses the DRT type 3 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

DRT type 3 (RT + RT)

Expression

Bacterial

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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