27,466 results
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Plasmid#129721PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-mScarlet-I tagged SeipinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-mScarlet-IExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)
Plasmid#129668PurposeA repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MaLionB
Plasmid#113910PurposeBlue ATP sensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMaLionB
ExpressionMammalianAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
SP-GFP-CD274-UTR
Plasmid#121142PurposeMammalian expression of GFP tagged CD274 with its own 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 with its own 3'UTR (CD274 Human)
ExpressionMammalianAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTagRFP
UseSynthetic BiologyAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB-CAGGS-dCas9
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
ExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS6-YFP-SMASh(AI)
Plasmid#111500PurposeExpresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYFP-SMASh(AI)
TagsHA epitope tag and SMASh tagExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q23
Plasmid#111726PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ23 (polyQ repeat)PromoterCMV and P10Available sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-ChroME-mRuby2-ST
Plasmid#108902PurposeCation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChroME-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4-GFP-Vinculin
Plasmid#107153Purposemammalian expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertVinculin (VCL Human)
Tags(His)10-GFPExpressionMammalianPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1 IGF1R-BirA(R118G)-HA
Plasmid#109232PurposeThis construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIGF1R (IGF1R Human)
TagsBirA(R118G) (highly promiscuous form) and HAExpressionMammalianPromoterT7Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-dCpf1-eBE
Plasmid#107688PurposeExpresses dLbCpf1-eBE in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdLbCpf1-eBE
ExpressionMammalianMutationdLbCpf1(D832A/E925A/D1148A)PromoterCMVAvailable sinceApril 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6 asyn E46K
Plasmid#105730PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein E46K (SNCA Human)
ExpressionMammalianMutationE46KPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA6 asyn A30P
Plasmid#105729PurposeMammalian expression of mutant human alpha synucleinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertalpha-synuclein A30P (SNCA Human)
ExpressionMammalianMutationA30PPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hygro(+)-mscGAS
Plasmid#102607PurposeMammalian expression of mouse cGASDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcGAS (Cgas Mouse)
ExpressionMammalianPromoterCMVAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
4xmt-mTurquoise2
Plasmid#98819PurposeIn vivo visualization of mitochondria (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmts
TagsmTurquoise2ExpressionMammalianPromoterCMVAvailable sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUC19-OCT4-T2A-NLS-EmGFP-P2A-Puro
Plasmid#89992PurposeDonor template for generation of OCT4-T2A-nEmGFP reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOCT4-T2A-NLS-EmGFP-P2A-Puro (POU5F1 Human)
UseCRISPRPromoternoneAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUGI-NLS
Plasmid#101091PurposeExpresses UGI in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUGI
TagsNLSExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLSSmOrange-mKate2
Plasmid#99868PurposeMeasure FRET between LSSmOrange and mKate2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLSSmOrange
TagsmKate2ExpressionMammalianPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by